T cell antigen receptor-mediated activation of phospholipase C requires tyrosine phosphorylation - PubMed (original) (raw)

. 1990 Mar 30;247(4950):1584-7.

doi: 10.1126/science.2138816.

Affiliations

• PMID: 2138816
• DOI: 10.1126/science.2138816

T cell antigen receptor-mediated activation of phospholipase C requires tyrosine phosphorylation

T Mustelin et al. Science. 1990.

Abstract

Triggering of the antigen-specific T cell receptor-CD3 complex (TCR-CD3) stimulates a rapid phospholipase C-mediated hydrolysis of inositol phospholipids, resulting in the production of second messengers and in T cell activation and proliferation. The role of tyrosine phosphorylation in these events was investigated with a tyrosine protein kinase (TPK) inhibitor, genistein. At doses that inhibited TPK activity and tyrosine phosphorylation of the TCR zeta subunit, but not phospholipase C activity, genistein prevented TCR-CD3-mediated phospholipase C activation, interleukin-2 receptor expression, and T cell proliferation. These findings indicate that tyrosine phosphorylation is an early and critical event that most likely precedes, and is a prerequisite for, inositol phospholipid breakdown during receptor-mediated T cell activation.

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