P granules extend the nuclear pore complex environment in the C. elegans germ line - PubMed (original) (raw)

P granules extend the nuclear pore complex environment in the C. elegans germ line

Dustin L Updike et al. J Cell Biol. 2011.

Abstract

The immortal and totipotent properties of the germ line depend on determinants within the germ plasm. A common characteristic of germ plasm across phyla is the presence of germ granules, including P granules in Caenorhabditis elegans, which are typically associated with the nuclear periphery. In C. elegans, nuclear pore complex (NPC)-like FG repeat domains are found in the VASA-related P-granule proteins GLH-1, GLH-2, and GLH-4 and other P-granule components. We demonstrate that P granules, like NPCs, are held together by weak hydrophobic interactions and establish a size-exclusion barrier. Our analysis of intestine-expressed proteins revealed that GLH-1 and its FG domain are not sufficient to form granules, but require factors like PGL-1 to nucleate the localized concentration of GLH proteins. GLH-1 is necessary but not sufficient for the perinuclear location of granules in the intestine. Our results suggest that P granules extend the NPC environment in the germ line and provide insights into the roles of the PGL and GLH family proteins.

PubMed Disclaimer

Figures

Figure 1.

Figure 1.

P granules create a size-exclusion barrier. (A) Model illustrating how P granules could extend the NPC environment through FG interactions. (B) C. elegans embryos from GFP::PGL-1 (green) worms injected with TRITC-labeled dextrans of different molecular weights (red).

Figure 2.

Figure 2.

Hydrophobic interactions are required for P-granule integrity. (A) Top, dissected GLH-1::GFP gonads exposed to hexanediol (HD), hexanetriol (HT), or egg buffer (Control). Bottom, normalized P-granule counts from the image series in A. Arrow indicates the time HD, HT, or control buffer was added. See

Video 1

for complete time series. (B–D) GLH-1::GFP granule averages (B), GFP::PGL-1 granule averages (C), and GFP::SPD-2 granule averages (D) for HD, HT, and control. Standard deviations for each condition are shown in blue, red, and yellow shaded bars. 20 dissected gonads were analyzed for each treatment of each GFP line.

Figure 3.

Figure 3.

Ectopic expression of P-granule components in the intestine. (A) Comma-stage embryo expressing GFP behind the elt-2 intestinal promoter. (B) FG repeats (blue triangles), FG domains (yellow), zinc fingers (gray), and DEAD-box helicase domains (red) in NPP-4, GLH-1, and GLH-3. (C–N) 10-µm projections of different ectopic expression constructs in the intestine of comma-stage embryos.

Figure 4.

Figure 4.

PGL-1 nucleates granule formation. (A) GFP-tagged and untagged PGL-1 expressed in the intestine. (B and D) GLH-1::mCherry (B) or GLH-3::mCherry (D) coexpressed with PGL-1::GFP in intestinal cells. (C and E) Young larvae expressing GLH-1::GFP (C) or the FG domain of GLH-1 tagged with GFP (E) in intestinal cells. The larvae In the right panels additionally express untagged PGL-1 in intestinal cells (transgenic PGL-1–expressing worms identified by myo-3::mCherry expression in body muscle).

Figure 5.

Figure 5.

GLH-1 is necessary but not sufficient for the perinuclear association of PGL granules. (A–D) Single XY plane of comma-stage embryos expressing tagged PGL-1 or GLH-1 constructs, immunostained for nuclear pores (red) and either PGL-1 or GLH-1 (green), as noted. (A) Embryo expressing PGL-1 in the intestine. Ectopic PGL-1 granules in the intestine (arrowhead), endogenous PGL-1 granules in the germ cells (arrow). (B) Embryo expressing 3X(FG)-GLH-1 in the intestine. Cytoplasmic granules (arrowhead), perinuclear granules (arrow). (C) hpl-2(tm1489) embryo expressing GLH-1 in the intestine. Cytoplasmic granules (arrowhead), perinuclear granules (arrow). (D) GLH-1 (left) and 3X(FG)-GLH-1 (middle) coexpressed with PGL-1 in intestinal cells. 3XFG from GLH-1 fused to PGL-1 (right). Ectopic PGL-1 granules in the intestine (arrowhead), endogenous PGL-1 granules in the germ cells (arrow). (E) Wild-type and hpl-2(tm1489) L1s grown at 26°C on control bacteria and glh-1(RNAi) bacteria and immunostained for GLH-1 and PGL-3. Germ cells (arrowheads). (F) Germ lines from wild-type worms grown from L1 to adulthood at 26°C on control bacteria and glh-1(RNAi) bacteria.

Similar articles

Cited by

References

    1. Amiri A., Keiper B.D., Kawasaki I., Fan Y., Kohara Y., Rhoads R.E., Strome S. 2001. An isoform of eIF4E is a component of germ granules and is required for spermatogenesis in C. elegans. Development. 128:3899–3912 - PMC - PubMed
    1. Aoyama J., Nakayama Y., Sugiyama D., Saburi S., Nadano D., Fukuda M.N., Yamaguchi N. 2005. Apical cell adhesion molecule, trophinin, localizes to the nuclear envelope. FEBS Lett. 579:6326–6332 10.1016/j.febslet.2005.10.012 - DOI - PubMed
    1. Batista P.J., Ruby J.G., Claycomb J.M., Chiang R., Fahlgren N., Kasschau K.D., Chaves D.A., Gu W., Vasale J.J., Duan S., et al. 2008. PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans. Mol. Cell. 31:67–78 10.1016/j.molcel.2008.06.002 - DOI - PMC - PubMed
    1. Blobel G. 1985. Gene gating: a hypothesis. Proc. Natl. Acad. Sci. USA. 82:8527–8529 10.1073/pnas.82.24.8527 - DOI - PMC - PubMed
    1. Bontems F., Stein A., Marlow F., Lyautey J., Gupta T., Mullins M.C., Dosch R. 2009. Bucky ball organizes germ plasm assembly in zebrafish. Curr. Biol. 19:414–422 10.1016/j.cub.2009.01.038 - DOI - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources