Scatter factor: molecular characteristics and effect on the invasiveness of epithelial cells - PubMed (original) (raw)

Scatter factor: molecular characteristics and effect on the invasiveness of epithelial cells

K M Weidner et al. J Cell Biol. 1990 Nov.

Abstract

The generation of invasiveness in transformed cells represents an essential step of tumor progression. We have previously shown that MDCK epithelial cells, which are deprived of intracellular adhesion by the addition of anti-Arc-1/uvomorulin antibodies, become invasive for collagen gels and embryonal heart tissue (Behrens, J., M. M. Mareel, F. M. Van Roy, and W. Birchmeier. 1989. J. Cell Biol. 108: 2435-2447.). Here we examined whether invasiveness is also induced by scatter factor, which is known to dissociate epithelial cells (Stoker, M., E. Gherardi, M. Perryman, and J. Gray. 1987. Nature (Lond.). 327:239-242.). Scatter factor was purified to homogeneity from conditioned medium of human fibroblasts by heparin-Sepharose chromatography, followed by cation exchange chromatography, gel filtration, or preparative SDS gel electrophoresis. We found that scatter factor represents a 92,000 mol wt glycoprotein which, apparently, is converted by limited proteolysis into disulfide-linked 62,000 and 34/32,000 mol wt subunits. Reversed phase HPLC and sequence analysis of tryptic peptides confirmed the suggested molecular structure, and revealed further that scatter factor exhibits sequence similarities to hepatocyte growth factor and to plasminogen. Purified scatter factor in fact induces the invasiveness into collagen matrices of MDCK epithelial cells, and induces or promotes the invasiveness of a number of human carcinoma cell lines. Apparently, the effect on the human cells depends on their respective degree of differentiation, i.e., cell lines with a less pronounced epithelial phenotype were more susceptible to the factor. Scatter factor does not seem to influence synthesis, steady-state level, and phosphorylation of the cell adhesion molecule Arc-1/uvomorulin. Thus, scatter factor represents a clearly defined molecular species which induces, in vitro, the progression of epithelial cells to a more motile, i.e., invasive phenotype.

PubMed Disclaimer

Similar articles

• The role of E-cadherin and scatter factor in tumor invasion and cell motility.
  Behrens J, Weidner KM, Frixen UH, Schipper JH, Sachs M, Arakaki N, Daikuhara Y, Birchmeier W. Behrens J, et al. EXS. 1991;59:109-26. doi: 10.1007/978-3-0348-7494-6_8. EXS. 1991. PMID: 1833225 Review.
• Purification of scatter factor, a fibroblast-derived basic protein that modulates epithelial interactions and movement.
  Gherardi E, Gray J, Stoker M, Perryman M, Furlong R. Gherardi E, et al. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5844-8. doi: 10.1073/pnas.86.15.5844. Proc Natl Acad Sci U S A. 1989. PMID: 2527367 Free PMC article.
• Purified scatter factor stimulates epithelial and vascular endothelial cell migration.
  Rosen EM, Meromsky L, Setter E, Vinter DW, Goldberg ID. Rosen EM, et al. Proc Soc Exp Biol Med. 1990 Oct;195(1):34-43. doi: 10.3181/00379727-195-43115. Proc Soc Exp Biol Med. 1990. PMID: 2144630
• Purification and characterization of scatter factor.
  Gherardi E, Coffer A. Gherardi E, et al. EXS. 1991;59:53-62. doi: 10.1007/978-3-0348-7494-6_4. EXS. 1991. PMID: 1833231 Review.

Cited by

• Hepatocyte growth factor/scatter factor-induced intracellular signalling.
  Stuart KA, Riordan SM, Lidder S, Crostella L, Williams R, Skouteris GG. Stuart KA, et al. Int J Exp Pathol. 2000 Feb;81(1):17-30. doi: 10.1046/j.1365-2613.2000.00138.x. Int J Exp Pathol. 2000. PMID: 10718861 Free PMC article. Review.
• c-MET expression in myofibroblasts: role in autocrine activation and prognostic significance in lung adenocarcinoma.
  Tokunou M, Niki T, Eguchi K, Iba S, Tsuda H, Yamada T, Matsuno Y, Kondo H, Saitoh Y, Imamura H, Hirohashi S. Tokunou M, et al. Am J Pathol. 2001 Apr;158(4):1451-63. doi: 10.1016/S0002-9440(10)64096-5. Am J Pathol. 2001. PMID: 11290563 Free PMC article.
• Growth factor signaling pathways as targets for prevention of epithelial carcinogenesis.
  Rho O, Kim DJ, Kiguchi K, Digiovanni J. Rho O, et al. Mol Carcinog. 2011 Apr;50(4):264-79. doi: 10.1002/mc.20665. Epub 2010 Jul 20. Mol Carcinog. 2011. PMID: 20648549 Free PMC article. Review.
• Hair follicle regeneration in skin grafts: current concepts and future perspectives.
  Mahjour SB, Ghaffarpasand F, Wang H. Mahjour SB, et al. Tissue Eng Part B Rev. 2012 Feb;18(1):15-23. doi: 10.1089/ten.teb.2011.0064. Epub 2011 Sep 1. Tissue Eng Part B Rev. 2012. PMID: 21883016 Free PMC article. Review.
• Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor.
  Wang R, Kobayashi R, Bishop JM. Wang R, et al. Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8425-30. doi: 10.1073/pnas.93.16.8425. Proc Natl Acad Sci U S A. 1996. PMID: 8710887 Free PMC article.

References

1. 1. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5600-4 - PubMed
2. 1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
3. 1. Biochem Biophys Res Commun. 1989 Sep 15;163(2):967-73 - PubMed
4. 1. Anticancer Res. 1987 Jul-Aug;7(4A):653-60 - PubMed
5. 1. Science. 1989 Aug 18;245(4919):752-5 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Ovid Technologies, Inc.
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database