Bordetella petrii infection with long-lasting persistence in human - PubMed (original) (raw)

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Bordetella petrii infection with long-lasting persistence in human

Alain Le Coustumier et al. Emerg Infect Dis. 2011 Apr.

Abstract

We report the repeated isolation of Bordetella petrii in the sputum of a 79-year-old female patient with diffuse bronchiectasis and persistence of the bacterium for >1 year. The patient was first hospitalized due to dyspnea, which developed into severe cough with purulent sputum that yielded B. petrii on culture. After this first episode, the patient was hospitalized an additional 4 times with bronchorrhea symptoms. The isolates collected were analyzed by using biochemical, genotypic, and proteomic tools. Expression of specific proteins was analyzed by using serum samples from the patient. The B. petrii isolates were compared with other B. petrii isolates collected from humans or the environment and with isolates of B. pertussis, B. parapertussis, B. bronchiseptica, and B. holmesii, obtained from human respiratory tract infections. Our observations indicate that B. petrii can persist in persons with chronic pulmonary obstructive disease as has been previously demonstrated for B. bronchiseptica.

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Figures

Figure 1

Figure 1

Genomic analysis of Bordetella petrii isolates chromosomal DNA profiles obtained after digestion with _Xba_I. Identity of the isolates is indicated.

Figure 2

Figure 2

Western blot analysis of 10 μL of bacterial suspension (1.8 × 1010 CFU/mL) loaded to a gel and subjected to electrophoresis. The proteins were transferred onto a nitrocellulose membrane, which was incubated in mouse or human serum as described in Materials and Methods. Serum samples used were convalescent-phase serum of the _Bordetella petrii_–infected patient (A), a pool of serum specimens from _B. pertussis_–infected patients (B), and a pool of serum specimens from _B. bronchiseptica–_infected patients (C). Lane 1, B. holmesii; lane 2, B. petrii FR3799; lane 3, B. petrii FR3891; lane 4, B. petrii FR3996; lane 5, B. petrii FR3497; lane 6, B. petrii KMBW; lane 7, B. pertussis 8132; lane 8, B. parapertussis 12822; lane 9, B. bronchiseptica RB50. Arrows indicate the proteins specifically recognized by the anti-serum.

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