Regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells in PEG-ECM hydrogels - PubMed (original) (raw)

. 2011 Jun;344(3):499-509.

doi: 10.1007/s00441-011-1153-2. Epub 2011 Apr 19.

Affiliations

• PMID: 21503601
• DOI: 10.1007/s00441-011-1153-2

Regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells in PEG-ECM hydrogels

Nathaniel S Hwang et al. Cell Tissue Res. 2011 Jun.

Abstract

Bone-marrow-derived mesenchymal stem cells (MSCs) are candidates for regeneration applications in musculoskeletal tissue such as cartilage and bone. Various soluble factors in the form of growth factors and cytokines have been widely studied for directing the chondrogenic and osteogenic differentiation of MSCs, but little is known about the way that the composition of extracellular matrix (ECM) components in three-dimensional microenvironments plays a role in regulating the differentiation of MSCs. To define whether ECM components influence the regulation of osteogenic and chondrogenic differentiation by MSCs, we encapsulated MSCs in poly-(ethylene glycol)-based (PEG-based) hydrogels containing exogenous type I collagen, type II collagen, or hyaluronic acids (HA) and cultured them for up to 6 weeks in chondrogenic medium containing transforming growth factor-β1 (10 ng/ml) or osteogenic medium. Actin cytoskeleton organization and cellular morphology were strongly dependent on which ECM components were added to the PEG-based hydrogels. Additionally, chondrogenic differentiation of MSCs was marginally enhanced in collagen-matrix-based hydrogels, whereas osteogenic differentiation, as measured by calcium accumulation, was induced in HA-containing hydrogels. Thus, the microenvironments created by exogenous ECM components seem to modulate the fate of MSC differentiation.

PubMed Disclaimer

Similar articles

• Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: influence of collagen type II extracellular matrix on MSC chondrogenesis.
  Bosnakovski D, Mizuno M, Kim G, Takagi S, Okumura M, Fujinaga T. Bosnakovski D, et al. Biotechnol Bioeng. 2006 Apr 20;93(6):1152-63. doi: 10.1002/bit.20828. Biotechnol Bioeng. 2006. PMID: 16470881
• Chondrogenic differentiation of human mesenchymal stem cells in collagen type I hydrogels.
  Nöth U, Rackwitz L, Heymer A, Weber M, Baumann B, Steinert A, Schütze N, Jakob F, Eulert J. Nöth U, et al. J Biomed Mater Res A. 2007 Dec 1;83(3):626-35. doi: 10.1002/jbm.a.31254. J Biomed Mater Res A. 2007. PMID: 17503531
• Evaluation of adult equine bone marrow- and adipose-derived progenitor cell chondrogenesis in hydrogel cultures.
  Kisiday JD, Kopesky PW, Evans CH, Grodzinsky AJ, McIlwraith CW, Frisbie DD. Kisiday JD, et al. J Orthop Res. 2008 Mar;26(3):322-31. doi: 10.1002/jor.20508. J Orthop Res. 2008. PMID: 17960654
• TGF-β1 regulates differentiation of bone marrow mesenchymal stem cells.
  Zhao L, Hantash BM. Zhao L, et al. Vitam Horm. 2011;87:127-41. doi: 10.1016/B978-0-12-386015-6.00042-1. Vitam Horm. 2011. PMID: 22127241 Review.
• Hydrogels for in vivo-like three-dimensional cellular studies.
  DeVolder R, Kong HJ. DeVolder R, et al. Wiley Interdiscip Rev Syst Biol Med. 2012 Jul-Aug;4(4):351-65. doi: 10.1002/wsbm.1174. Epub 2012 May 21. Wiley Interdiscip Rev Syst Biol Med. 2012. PMID: 22615143 Review.

Cited by

• Lipid Coated Microbubbles and Low Intensity Pulsed Ultrasound Enhance Chondrogenesis of Human Mesenchymal Stem Cells in 3D Printed Scaffolds.
  Aliabouzar M, Zhang LG, Sarkar K. Aliabouzar M, et al. Sci Rep. 2016 Nov 24;6:37728. doi: 10.1038/srep37728. Sci Rep. 2016. PMID: 27883051 Free PMC article.
• Hyaluronic acid-binding scaffold for articular cartilage repair.
  Unterman SA, Gibson M, Lee JH, Crist J, Chansakul T, Yang EC, Elisseeff JH. Unterman SA, et al. Tissue Eng Part A. 2012 Dec;18(23-24):2497-506. doi: 10.1089/ten.TEA.2011.0711. Epub 2012 Aug 14. Tissue Eng Part A. 2012. PMID: 22724901 Free PMC article.
• Polymers in Cartilage Defect Repair of the Knee: Current Status and Future Prospects.
  Jeuken RM, Roth AK, Peters RJRW, Van Donkelaar CC, Thies JC, Van Rhijn LW, Emans PJ. Jeuken RM, et al. Polymers (Basel). 2016 Jun 4;8(6):219. doi: 10.3390/polym8060219. Polymers (Basel). 2016. PMID: 30979313 Free PMC article. Review.
• Comparative effect of physicomechanical and biomolecular cues on zone-specific chondrogenic differentiation of mesenchymal stem cells.
  Moeinzadeh S, Pajoum Shariati SR, Jabbari E. Moeinzadeh S, et al. Biomaterials. 2016 Jun;92:57-70. doi: 10.1016/j.biomaterials.2016.03.034. Epub 2016 Mar 23. Biomaterials. 2016. PMID: 27038568 Free PMC article.
• Mechanisms and Microenvironment Investigation of Cellularized High Density Gradient Collagen Matrices via Densification.
  Novak T, Seelbinder B, Twitchell CM, van Donkelaar CC, Voytik-Harbin SL, Neu CP. Novak T, et al. Adv Funct Mater. 2016 Apr 25;26(16):2617-2628. doi: 10.1002/adfm.201503971. Epub 2016 Feb 19. Adv Funct Mater. 2016. PMID: 27346992 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Springer

• Other Literature Sources

  • The Lens - Patent Citations Database