Aminoethoxydiphenyl borate and flufenamic acid inhibit Ca2+ influx through TRPM2 channels in rat dorsal root ganglion neurons activated by ADP-ribose and rotenone - PubMed (original) (raw)
Aminoethoxydiphenyl borate and flufenamic acid inhibit Ca2+ influx through TRPM2 channels in rat dorsal root ganglion neurons activated by ADP-ribose and rotenone
Mustafa Nazıroğlu et al. J Membr Biol. 2011 May.
Abstract
Exposure to oxidative stress causes health problems, including sensory neuron neuropathy and pain. Rotenone is a toxin used to generate intracellular oxidative stress in neurons. However, the mechanism of toxicity in dorsal root ganglion (DRG) neurons has not been characterized. Melastatin-like transient receptor potential 2 (TRPM2) channel activation and inhibition in response to oxidative stress, ADP-ribose (ADPR), flufenamic acid (FFA) and 2-aminoethoxydiphenyl borate (2-APB) in DRG neurons are also not clear. We tested the effects of FFA and 2-APB on ADPR and rotenone-induced TRPM2 cation channel activation in DRG neurons of rats. DRG neurons were freshly isolated from rats and studied with the conventional whole-cell patch-clamp technique. Rotenone, FFA and 2-APB were extracellularly added through the patch chamber, and ADPR was applied intracellularly through the patch pipette. TRPM2 cation currents were consistently induced by ADPR and rotenone. Current densities of the neurons were higher in the ADPR and rotenone groups than in control. The time courses (gating times) in the neurons were longer in the rotenone than in the ADPR group. ADPR and rotenone-induced TRPM2 currents were totally blocked by 2-APB and partially blocked by FFA. In conclusion, TRPM2 channels were constitutively activated by ADPR and rotenone, and 2-APB and FFA induced an inhibitory effect on TRPM2 cation channel currents in rat DRG neurons. Since oxidative stress is a common feature of neuropathic pain and diseases of sensory neurons, the present findings have broad application to the etiology of neuropathic pain and diseases of DRG neurons.
Similar articles
- TRPM2 channel membrane currents in primary rat megakaryocytes were activated by the agonist ADP-ribose but not oxidative stress.
Nazıroğlu M. Nazıroğlu M. J Membr Biol. 2011 May;241(2):51-7. doi: 10.1007/s00232-011-9356-8. Epub 2011 Apr 22. J Membr Biol. 2011. PMID: 21512734 - Glutathione modulates Ca(2+) influx and oxidative toxicity through TRPM2 channel in rat dorsal root ganglion neurons.
Nazıroğlu M, Özgül C, Çiğ B, Doğan S, Uğuz AC. Nazıroğlu M, et al. J Membr Biol. 2011 Aug;242(3):109-18. doi: 10.1007/s00232-011-9382-6. Epub 2011 Jul 12. J Membr Biol. 2011. PMID: 21748272 - TRPM2 channel protective properties of N-acetylcysteine on cytosolic glutathione depletion dependent oxidative stress and Ca2+ influx in rat dorsal root ganglion.
Özgül C, Nazıroğlu M. Özgül C, et al. Physiol Behav. 2012 May 15;106(2):122-8. doi: 10.1016/j.physbeh.2012.01.014. Epub 2012 Jan 24. Physiol Behav. 2012. PMID: 22300897 - TRPM2 cation channels, oxidative stress and neurological diseases: where are we now?
Nazıroğlu M. Nazıroğlu M. Neurochem Res. 2011 Mar;36(3):355-66. doi: 10.1007/s11064-010-0347-4. Epub 2010 Dec 8. Neurochem Res. 2011. PMID: 21140288 Review. - New molecular mechanisms on the activation of TRPM2 channels by oxidative stress and ADP-ribose.
Naziroğlu M. Naziroğlu M. Neurochem Res. 2007 Nov;32(11):1990-2001. doi: 10.1007/s11064-007-9386-x. Epub 2007 Jun 12. Neurochem Res. 2007. PMID: 17562166 Review.
Cited by
- Effect of Long-Term Adaptation to Cold and Short-Term Cooling on the Expression of the TRPM2 Ion Channel Gene in the Hypothalamus of Rats.
Evtushenko AA, Voronova IP, Kozyreva TV. Evtushenko AA, et al. Curr Issues Mol Biol. 2023 Jan 20;45(2):1002-1011. doi: 10.3390/cimb45020065. Curr Issues Mol Biol. 2023. PMID: 36826010 Free PMC article. - Pharmacological Modulation of TRPM2 Channels via PARP Pathway Leads to Neuroprotection in MPTP-induced Parkinson's Disease in Sprague Dawley Rats.
Vaidya B, Kaur H, Thapak P, Sharma SS, Singh JN. Vaidya B, et al. Mol Neurobiol. 2022 Mar;59(3):1528-1542. doi: 10.1007/s12035-021-02711-4. Epub 2022 Jan 8. Mol Neurobiol. 2022. PMID: 34997907 - Involvement of TRPM2 in the Neurobiology of Experimental Migraine: Focus on Oxidative Stress and Apoptosis.
Yazğan Y, Nazıroğlu M. Yazğan Y, et al. Mol Neurobiol. 2021 Nov;58(11):5581-5601. doi: 10.1007/s12035-021-02503-w. Epub 2021 Aug 9. Mol Neurobiol. 2021. PMID: 34370177 - Transient Receptor Potential Channels as an Emerging Target for the Treatment of Parkinson's Disease: An Insight Into Role of Pharmacological Interventions.
Vaidya B, Sharma SS. Vaidya B, et al. Front Cell Dev Biol. 2020 Nov 20;8:584513. doi: 10.3389/fcell.2020.584513. eCollection 2020. Front Cell Dev Biol. 2020. PMID: 33330461 Free PMC article. Review. - Curcumin Attenuates Hypoxia-Induced Oxidative Neurotoxicity, Apoptosis, Calcium, and Zinc Ion Influxes in a Neuronal Cell Line: Involvement of TRPM2 Channel.
Armağan HH, Nazıroğlu M. Armağan HH, et al. Neurotox Res. 2021 Jun;39(3):618-633. doi: 10.1007/s12640-020-00314-w. Epub 2020 Nov 19. Neurotox Res. 2021. PMID: 33211286
References
- Br J Pharmacol. 2008 Mar;153(6):1324-30 - PubMed
- Neurochem Res. 2008 Jul;33(7):1256-62 - PubMed
- Neuropharmacology. 2003 Apr;44(5):642-52 - PubMed
- Gene Expr Patterns. 2010 Jan;10(1):65-74 - PubMed
- NeuroRx. 2005 Jul;2(3):484-94 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous