Release of RNA polymerase from vero cell mitochondria after herpes simplex virus type 1 infection - PubMed (original) (raw)

Release of RNA polymerase from vero cell mitochondria after herpes simplex virus type 1 infection

T Tsurumi et al. J Virol. 1990 Jan.

Abstract

Infection of Vero cells with herpes simplex virus type 1 results in the appearance in soluble extracts of a DNA primase activity. The partially purified enzyme, Mr, approximately 100,000, is identical in resistance to alpha-amanitin, pH profile, Mg2+ dependence, salt sensitivity, and KmATP to the catalytic core of Vero cell mitochondrial RNA polymerase. Moreover, the products synthesized are those expected of an RNA polymerase rather than a DNA primase. Inasmuch as the enzyme is not present in soluble extracts of uninfected Vero cells, we presume that the specific appearance of RNA polymerase in extracts of herpesvirus-infected cells results from infection-induced disruption of the mitochondrial membrane, followed by release of the enzyme into the cytosol.

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References

    1. J Virol. 1983 Jan;45(1):354-66 - PubMed
    1. J Biol Chem. 1984 Feb 10;259(3):1854-65 - PubMed
    1. J Biol Chem. 1985 Sep 25;260(21):11530-5 - PubMed
    1. Cell. 1986 Jun 20;45(6):817-25 - PubMed
    1. J Biol Chem. 1986 Aug 5;261(22):10340-7 - PubMed

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