Infection of cultured central nervous system neurons with a defective herpes simplex virus 1 vector results in stable expression of Escherichia coli beta-galactosidase - PubMed (original) (raw)

Infection of cultured central nervous system neurons with a defective herpes simplex virus 1 vector results in stable expression of Escherichia coli beta-galactosidase

A I Geller et al. Proc Natl Acad Sci U S A. 1990 Feb.

Abstract

We have developed a defective herpes simplex virus (HSV) vector system that permits the introduction of virtually any gene into mammalian central nervous system neurons. The prototype vector, pHSVlac, contains a transcription unit that places the Escherichia coli lacZ gene under the control of the HSV-1 immediate early 4/5 promoter. pHSVlac was propagated using the HSV-1 temperature-sensitive mutant ts K as helper virus. Infection of rat neurons in primary culture derived from various regions throughout the central nervous system, including spinal cord, cerebellum, thalamus, basal ganglia, hippocampus, occipital cortex, temporal cortex, and frontal cortex, resulted in stable expression of high levels of beta-galactosidase for at least 2 weeks, without cell damage. Since other genes can be expressed from pHSVlac, HSV-1 vectors may prove useful for delivery of genes into central nervous system neurons for studies on nervous system physiology or to perform gene therapy for neurological conditions.

PubMed Disclaimer

Cited by

Of Molecules and Mechanisms.
Benson DL. Benson DL. J Neurosci. 2020 Jan 2;40(1):81-88. doi: 10.1523/JNEUROSCI.0743-19.2019. Epub 2019 Oct 19. J Neurosci. 2020. PMID: 31630114 Free PMC article. Review.
Replication-defective HSV-1 effectively targets trigeminal ganglion and inhibits viral pathopoiesis by mediating interferon gamma expression in SH-SY5Y cells.
Xu K, Liu XN, Zhang HB, An N, Wang Y, Zhang ZC, Wang YN. Xu K, et al. J Mol Neurosci. 2014 May;53(1):78-86. doi: 10.1007/s12031-013-0199-x. Epub 2013 Dec 18. J Mol Neurosci. 2014. PMID: 24347277
Augmentation of anti-tumor responses of adoptively transferred CD8+T cells in the lymphopenic setting by HSV amplicon transduction.
Nechushtan H, Pham D, Zhang Y, Morgensztern D, Yi KH, Shin SU, Federoff HJ, Bowers WJ, Tolba KA, Rosenblatt JD. Nechushtan H, et al. Cancer Immunol Immunother. 2008 May;57(5):663-75. doi: 10.1007/s00262-007-0405-1. Epub 2007 Oct 19. Cancer Immunol Immunother. 2008. PMID: 17952436 Free PMC article.
Complement depletion facilitates the infection of multiple brain tumors by an intravascular, replication-conditional herpes simplex virus mutant.
Ikeda K, Wakimoto H, Ichikawa T, Jhung S, Hochberg FH, Louis DN, Chiocca EA. Ikeda K, et al. J Virol. 2000 May;74(10):4765-75. doi: 10.1128/jvi.74.10.4765-4775.2000. J Virol. 2000. PMID: 10775615 Free PMC article.
Overexpression of glyoxalase-I in bovine endothelial cells inhibits intracellular advanced glycation endproduct formation and prevents hyperglycemia-induced increases in macromolecular endocytosis.
Shinohara M, Thornalley PJ, Giardino I, Beisswenger P, Thorpe SR, Onorato J, Brownlee M. Shinohara M, et al. J Clin Invest. 1998 Mar 1;101(5):1142-7. doi: 10.1172/JCI119885. J Clin Invest. 1998. PMID: 9486985 Free PMC article.

References

1. Curr Top Microbiol Immunol. 1975;70:31-50 - PubMed
1. Science. 1983 Nov 18;222(4625):809-14 - PubMed
1. Proc R Soc Lond B Biol Sci. 1977 Jul 28;198(1130):1-59 - PubMed
1. Virology. 1978 Jun 1;87(1):34-41 - PubMed
1. Cell. 1979 Apr;16(4):777-85 - PubMed

Infection of cultured central nervous system neurons with a defective herpes simplex virus 1 vector results in stable expression of Escherichia coli beta-galactosidase - PubMed (original) (raw)