Identification of a new membrane-associated polypeptide specified by the coronavirus infectious bronchitis virus - PubMed (original) (raw)
Identification of a new membrane-associated polypeptide specified by the coronavirus infectious bronchitis virus
A R Smith et al. J Gen Virol. 1990 Jan.
Abstract
Nucleotide sequences from the third open reading frame of mRNA D (D3) of infectious bronchitis virus (IBV) were expressed in bacteria as part of a fusion protein with beta-galactosidase. Antiserum raised in rabbits against this fusion protein immunoprecipitated from IBV-infected chick kidney or Vero cells a polypeptide of 12.4K, the size expected for a D3-encoded product. The D3 polypeptide is apparently non-glycosylated, and appears to be associated with the membrane fraction of infected cells, as judged by cell fractionation and immunofluorescence.
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