Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen - PubMed (original) (raw)

Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen

Tan Liu et al. Biomed Opt Express. 2011.

Abstract

We proposed to measure the metabolic rate of oxygen (MRO(2)) in small animals in vivo using a multimodal imaging system that combines laser-scanning optical-resolution photoacoustic microscopy (LSOR-PAM) and spectral-domain optical coherence tomography (SD-OCT). We first tested the capability of the multimodal system to measure flow rate in a phantom made of two capillary tubes of different diameters. We then demonstrated the capability of measuring MRO(2) by imaging two parallel vessels selected from the ear of a Swiss Webster mouse. The hemoglobin oxygen saturation (sO(2)) and the vessel diameter were measured by the LSOR-PAM and the blood flow velocity was measured by the SD-OCT, from which blood flow rate and MRO(2) were further calculated. The measured blood flow rates in the two vessels agreed with each other.

Keywords: (110.5120) Photoacoustic imaging; (170.2655) Functional monitoring and imaging; (170.4500) Optical coherence tomography.

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Figures

Fig. 1

Fig. 1

Schematics of the combined LSOR-PAM and SD-OCT. PD: photodiode; 2 × 2: 2 × 2 single-mode optical fiber coupler; HM: hot mirror; GM: 2D galvanometer; OBJ: objective lens; AMP: amplifier; UT: ultrasonic transducer; WT: water tank; SLED: superluminescent emitting diode; Ref: OCT reference arm.

Fig. 2

Fig. 2

Flow phantom imaging results. (a) LSOR-PAM image of the tubes (mean intensity projection); (b) PA amplitude profile along the dashed line in panel a; (c) flow velocity measured by Doppler OCT at the cross section marked in panel a; (d) the flow velocity profile at locations marked by the two dash lines in panel c. Bar: 250 _µ_m.

Fig. 3

Fig. 3

Multimodal anatomic imaging of a mouse ear. (a) LSOR-PAM projection image of an artery-vein pair; (b) LSOR-PAM B-Scan image acquired at the 570-nm optical wavelength at the location marked by the dashed line in panel a; (c) OCT B-Scan image acquired at the same location. A: Artery; V: Vein; Ep, epidermis; De, dermis; Ca, cartilaginous backbone; Bar: 100 _µ_m.

Fig. 4

Fig. 4

Multimodal functional imaging. (a) Imaged sO2 using LSOR-PAM; (b) Imaged blood flow velocity using SD-OCT. Bar: 100 _µ_m.

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