Impact of medicated feed on the development of antimicrobial resistance in bacteria at integrated pig-fish farms in Vietnam - PubMed (original) (raw)

Impact of medicated feed on the development of antimicrobial resistance in bacteria at integrated pig-fish farms in Vietnam

Son Thi Thanh Dang et al. Appl Environ Microbiol. 2011 Jul.

Abstract

Integrated livestock-fish aquaculture utilizes animal excreta, urine, and feed leftovers as pond fertilizers to enhance the growth of plankton and other microorganisms eaten by the fish. However, antimicrobial-resistant bacteria may be transferred and develop in the pond due to selective pressure from antimicrobials present in animal feed, urine, and feces. In an experimental pig-fish farm located in periurban Hanoi, Vietnam, nine piglets were provided feed containing 5 μg of tetracycline (TET)/kg pig weight/day and 0.45 μg of enrofloxacin (ENR)/kg pig weight/day during the second and fourth (last) months of the experiment. The aim of this study was to determine the association between the provision of pig feed with antimicrobials and the development of antimicrobial resistance, as measured in a total of 520 Escherichia coli and 634 Enterococcus strains isolated from pig manure and water-sediment pond samples. MIC values for nalidixic acid (NAL) and ENR showed that E. coli and Enterococcus spp. overall exhibited significant higher frequencies of resistance toward NAL and ENR during the 2 months when pigs were administered feed with antimicrobials, with frequencies reaching 60 to 80% in both water-sediment and manure samples. TET resistance for both indicators was high (>80%) throughout the study period, which indicates that TET-resistant E. coli and Enterococcus spp. were present in the piglets before the initiation of the experiment. PCR-based identification showed similar relative occurrences of Enterococcus faecium, Enterococcus faecalis, and other Enterococcus spp. in the water-sediment and manure samples, suggesting that Enterococcus spp. isolated in the ponds originated mainly from the pig manure. The development of antimicrobial resistance in integrated animal husbandry-fish farms and possible transfers and the impact of such resistance on food safety and human health should be further assessed.

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Figures

Fig. 1.

Schematic illustration of an experimental integrated pig-fish farm showing sample types and sites.

Fig. 2.

The frequency (percentage) of tetracycline- and quinolone-resistant E. coli and Enterococcus spp. isolated from pig manure during four monthly samplings. The asterisks placed above the bars indicate significant differences in average resistance compared to that from the previous month.

Fig. 3.

The frequency (percentage) of tetracycline- and quinolone-resistant E. coli and Enterococcus spp. isolated from water-sediment samples during four monthly samplings. The asterisks placed above the bars indicate significant differences in average resistance compared to that from the previous month.

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References

1. Alderman D. J., Hastings T. S. 1998. Antibiotic use in aquaculture: development of antibiotic resistance potential for consumer health risks. Int. J. Food Sci. Tech. 33:139–155
1. Alexander T. W., et al. 2008. Effect of subtherapeutic administration of antibiotics on the prevalence of antibiotic-resistant Escherichia coli bacteria in feedlot cattle. Appl. Environ. Microbiol. 74:4405–4416 - PMC - PubMed
1. Reference deleted.
1. Cavaco L. M., et al. 2008. Prevalence of quinolone resistance mechanisms and associations to minimum inhibitory concentrations in quinolone-resistant Escherichia coli isolated from humans and swine in Denmark. Microb. Drug Resist. 14:163–169 - PubMed
1. CLSI/NCCLS 2001. Performance standards for antimicrobial susceptibility testing: 11th informational supplement. CLSI/NCCLS, Wayne, PA.

Impact of medicated feed on the development of antimicrobial resistance in bacteria at integrated pig-fish farms in Vietnam - PubMed (original) (raw)