In vitro 3D collective sprouting angiogenesis under orchestrated ANG-1 and VEGF gradients - PubMed (original) (raw)
. 2011 Jul 7;11(13):2175-81.
doi: 10.1039/c1lc20039a. Epub 2011 May 26.
Affiliations
- PMID: 21617793
- DOI: 10.1039/c1lc20039a
In vitro 3D collective sprouting angiogenesis under orchestrated ANG-1 and VEGF gradients
Yoojin Shin et al. Lab Chip. 2011.
Abstract
Sprouting angiogenesis requires a coordinated guidance from a variety of angiogenic factors. Here, we have developed a unique hydrogel incorporating microfluidic platform which mimics the physiological microenvironment in 3D under a precisely orchestrated gradient of soluble angiogenic factors, VEGF and ANG-1. The system enables the quantified investigation in chemotactic response of endothelial cells during the collective angiogenic sprouting process. While the presence of a VEGF gradient alone was sufficient in inducing a greater number of tip cells, addition of ANG-1 to the VEGF gradient enhanced the number of tip cells that are attached to collectively migrated stalk cells. The chemotactic response of tip cells attracted by the VEGF gradient and the stabilizing role of ANG-1 were morphologically investigated, elucidating the 3D co-operative migration of tip and stalk cells as well as their structures. We found that ANG-1 enhanced the connection of the stalk cells with the tip cells, and then the direct connection regulated the morphogenesis and/or life cycle of stalk cells.
This journal is © The Royal Society of Chemistry 2011
Comment in
- Regen Med. 2011 Sep;6(5):551-4
Similar articles
- Perfused 3D angiogenic sprouting in a high-throughput in vitro platform.
van Duinen V, Zhu D, Ramakers C, van Zonneveld AJ, Vulto P, Hankemeier T. van Duinen V, et al. Angiogenesis. 2019 Feb;22(1):157-165. doi: 10.1007/s10456-018-9647-0. Epub 2018 Aug 31. Angiogenesis. 2019. PMID: 30171498 Free PMC article. - RhoA mediates flow-induced endothelial sprouting in a 3-D tissue analogue of angiogenesis.
Song JW, Daubriac J, Tse JM, Bazou D, Munn LL. Song JW, et al. Lab Chip. 2012 Dec 7;12(23):5000-6. doi: 10.1039/c2lc40389g. Lab Chip. 2012. PMID: 23073300 Free PMC article. - Contextual role for angiopoietins and TGFbeta1 in blood vessel stabilization.
Ramsauer M, D'Amore PA. Ramsauer M, et al. J Cell Sci. 2007 May 15;120(Pt 10):1810-7. doi: 10.1242/jcs.003533. J Cell Sci. 2007. PMID: 17502485 - Dynamics of endothelial cell behavior in sprouting angiogenesis.
Eilken HM, Adams RH. Eilken HM, et al. Curr Opin Cell Biol. 2010 Oct;22(5):617-25. doi: 10.1016/j.ceb.2010.08.010. Curr Opin Cell Biol. 2010. PMID: 20817428 Review. - How do endothelial cells orientate?
Gerhardt H, Betsholtz C. Gerhardt H, et al. EXS. 2005;(94):3-15. doi: 10.1007/3-7643-7311-3_1. EXS. 2005. PMID: 15617467 Review.
Cited by
- Vascularized platforms for investigating cell communication via extracellular vesicles.
Kim J, Ro J, Cho YK. Kim J, et al. Biomicrofluidics. 2024 Sep 23;18(5):051504. doi: 10.1063/5.0220840. eCollection 2024 Sep. Biomicrofluidics. 2024. PMID: 39323481 Review. - Organ-on-a-Chip: Ubi sumus? Fundamentals and Design Aspects.
Morais AS, Mendes M, Cordeiro MA, Sousa JJ, Pais AC, Mihăilă SM, Vitorino C. Morais AS, et al. Pharmaceutics. 2024 May 2;16(5):615. doi: 10.3390/pharmaceutics16050615. Pharmaceutics. 2024. PMID: 38794277 Free PMC article. - Tissue-Engineered Microvessels: A Review of Current Engineering Strategies and Applications.
Zhao N, Pessell AF, Zhu N, Searson PC. Zhao N, et al. Adv Healthc Mater. 2024 Aug;13(21):e2303419. doi: 10.1002/adhm.202303419. Epub 2024 May 9. Adv Healthc Mater. 2024. PMID: 38686434 Review. - Matrix Resistance Toward Proteolytic Cleavage Controls Contractility-Dependent Migration Modes During Angiogenic Sprouting.
Weiß MS, Trapani G, Long H, Trappmann B. Weiß MS, et al. Adv Sci (Weinh). 2024 May;11(19):e2305947. doi: 10.1002/advs.202305947. Epub 2024 Mar 13. Adv Sci (Weinh). 2024. PMID: 38477409 Free PMC article. - Microneedle array facilitates hepatic sinusoid construction in a large-scale liver-acinus-chip microsystem.
Li S, Li C, Khan MI, Liu J, Shi Z, Gao D, Qiu B, Ding W. Li S, et al. Microsyst Nanoeng. 2023 Jun 7;9:75. doi: 10.1038/s41378-023-00544-w. eCollection 2023. Microsyst Nanoeng. 2023. PMID: 37303831 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous