A reverse transcriptase-related protein mediates phage resistance and polymerizes untemplated DNA in vitro - PubMed (original) (raw)

Alignment of AbiK sequence with group II intron RTs and RNA-dependent RNA polymerases. An amino acid alignment is shown for the AbiK protein, four closely related proteins, and representative group II intron RTs. Above AbiK is the consensus sequence for the seven domains of RNA-dependent RNA polymerases (RdRPs), derived from the Pfam database (31). Only the seven domains conserved among RT proteins are shown; full alignments are available in

Supplementary Figure S1

. Asterisks directly above AbiK sequence indicate residues mutated in this study, while the three outlined asterisks below the group II intron sequences mark the putative active site aspartate residues. Bracketed numbers are amino acids not shown in the alignment. Sequences are: AbiK (Lactococcus lactis, gi|32455447), S.a.1 (Staphylococcus aureus, gi|17227201), S.a.2 (Staphylococcus aureus, gi|24636606), S.e. (Staphylococcus epidermidis, gi|281296383), G.e. (Granulicatella elegans, gi|260157297), E.c.I.4 (Escherichia coli, class A; gi|6009435), B.h.I1 (Bacillus halodurans, class C; gi|10172711), Sr.me.I1 (Sinorhizobium meliloti, class D; gi|4586216), A.v.I5 (Azotobacter vinelandii, class E; gi|226719487), Pe.th.I2 (Pelotomaculum thermopropionicum, class F; gi|146274844), C.d.I1 (Clostridium difficile, class B; gi|1418413), E.c.I5 (Escherichia coli, class CL; gi|188574179), L.l.I1 (Lactococcus lactis, class ML; gi|125624188).