Conditioned medium from activated rat macrophages and the recombinant factors, IL-1 beta and GM-CSF, enhance the accessory activity of dendritic cells - PubMed (original) (raw)

Conditioned medium from activated rat macrophages and the recombinant factors, IL-1 beta and GM-CSF, enhance the accessory activity of dendritic cells

W E Bowers et al. Immunobiology. 1990 Jun.

Abstract

Low density lymph node cells (LD-LNC; 5% of total unfractionated LNC) contain 95% of the accessory activity required for responses of T lymphocytes to mitogens. Significantly greater responses to mitogens occur when T lymphocytes are added to LD-LNC that have been exposed overnight to silica, in comparison to responses occurring with LD-LNC incubated without silica. Conditioned medium (CM) from silica-treated LD-LNC is itself able to mediate enhanced responses; i.e., when LD-LNC are exposed overnight to CM alone and mitogen-treated T lymphocytes added the next day. The enhancing activity found in CM from LD-LNC exposed to silica is produced by macrophages; however, their low accessory activity is not enhanced by CM. In contrast, dendritic cells isolated from LD-LNC exposed to silica or to CM show significantly increased accessory activity, but dendritic cells do not produce the enhancing activity found in CM. CM lacks IL-2 activity and does not have any effect on the responses of untreated or mitogen-treated T lymphocytes alone. Thus, macrophages produce the enhancing activity and dendritic cells respond to it. Maximum enhancement of dendritic cell accessory activity requires overnight exposure to CM; once induced, accessory activity is not further modulated after continued incubation in the presence or absence of CM. LD-LNC, adherent peritoneal exudate cells, and adherent thioglycollate-induced peritoneal exudate cells produce enhancing activity after exposure to silica, LPS, and silica plus LPS. After gel filtration of a CM produced by silica plus LPS, enhancing activity shows a broad molecular weight distribution between 20 and 55 kD. IL-1 is present in CM and shows a more narrow molecular weight distribution that falls within the lower molecular weight range for enhancing activity. Silica treatment by itself produces CM containing little IL-1, but abundant enhancing activity; gel filtration of this CM shows that the distribution of enhancing activity is confined more narrowly to the higher molecular weight range, suggesting that IL-1 is one of several factors that enhances the accessory activity of dendritic cells. Recombinant human IL-1 beta does have enhancing activity, but of the other recombinant factors tested only mouse GM-CSF also has enhancing activity. Human IL-1 alpha, tumor necrosis factor alpha, IL-4, rat IL-3 and rat IFN-gamma, as well as L cell-conditioned medium containing M-CSF, lack enhancing activity.

PubMed Disclaimer

Cited by

Heterogeneity of dendritic cells in human superficial lymph node: in vitro maturation of immature dendritic cells into mature or activated interdigitating reticulum cells.
Takahashi K, Asagoe K, Zaishun J, Yanai H, Yoshino T, Hayashi K, Akagi T. Takahashi K, et al. Am J Pathol. 1998 Sep;153(3):745-55. doi: 10.1016/S0002-9440(10)65618-0. Am J Pathol. 1998. PMID: 9736025 Free PMC article.
High stimulatory activity of dendritic cells from diabetes-prone BioBreeding/Worcester rats exposed to macrophage-derived factors.
Tafuri A, Bowers WE, Handler ES, Appel M, Lew R, Greiner D, Mordes JP, Rossini AA. Tafuri A, et al. J Clin Invest. 1993 May;91(5):2040-8. doi: 10.1172/JCI116426. J Clin Invest. 1993. PMID: 8486773 Free PMC article.
Downregulation of the antigen presenting cell function(s) of pulmonary dendritic cells in vivo by resident alveolar macrophages.
Holt PG, Oliver J, Bilyk N, McMenamin C, McMenamin PG, Kraal G, Thepen T. Holt PG, et al. J Exp Med. 1993 Feb 1;177(2):397-407. doi: 10.1084/jem.177.2.397. J Exp Med. 1993. PMID: 8426110 Free PMC article.
Interstitial lung macrophages interact with dendritic cells to present antigenic peptides derived from particulate antigens to T cells.
Gong JL, McCarthy KM, Rogers RA, Schneeberger EE. Gong JL, et al. Immunology. 1994 Mar;81(3):343-51. Immunology. 1994. PMID: 8206508 Free PMC article.
Secretory and accessory cell functions of the alveolar macrophage.
Miller K, Hudspith BN, Meredith C. Miller K, et al. Environ Health Perspect. 1992 Jul;97:85-9. doi: 10.1289/ehp.929785. Environ Health Perspect. 1992. PMID: 1396471 Free PMC article.

Conditioned medium from activated rat macrophages and the recombinant factors, IL-1 beta and GM-CSF, enhance the accessory activity of dendritic cells - PubMed (original) (raw)