Identification of a receptor for protein import into mitochondria - PubMed (original) (raw)

. 1990 Oct 4;347(6292):444-9.

doi: 10.1038/347444a0.

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Identification of a receptor for protein import into mitochondria

D Pain et al. Nature. 1990.

Abstract

Anti-idiotypic antibodies, prepared using a chemically synthesized signal peptide of a mitochondrial precursor protein, recognized a mitochondrial integral membrane protein (p32). Fab fragments derived from both anti-idiotypic antibodies and monospecific antibodies against purified p32 inhibited protein import into mitochondria. Moreover, anti-p32 antibodies specifically immunoprecipitated a precursor-p32 complex after detergent solubilization of mitochondria. Immunoelectron microscopy and subfractionation of mitochondria indicate that p32 is located in contact sites between the outer and inner mitochondrial membranes.

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