Bone marrow mononuclear cells reduce seizure frequency and improve cognitive outcome in chronic epileptic rats - PubMed (original) (raw)
. 2011 Aug 15;89(7-8):229-34.
doi: 10.1016/j.lfs.2011.06.006. Epub 2011 Jun 22.
Affiliations
- PMID: 21718708
- DOI: 10.1016/j.lfs.2011.06.006
Free article
Bone marrow mononuclear cells reduce seizure frequency and improve cognitive outcome in chronic epileptic rats
Gianina Teribele Venturin et al. Life Sci. 2011.
Free article
Abstract
Aims: Epilepsy affects 0.5-1% of the world's population, and approximately a third of these patients are refractory to current medication. Given their ability to proliferate, differentiate and regenerate tissues, stem cells could restore neural circuits lost during the course of the disease and reestablish the physiological excitability of neurons. This study verified the therapeutic potential of bone marrow mononuclear cells (BMMCs) on seizure control and cognitive impairment caused by experimentally induced epilepsy.
Main methods: Status epilepticus (SE) was induced by lithium-pilocarpine injection and controlled with diazepam 90 min after SE onset. Lithium-pilocarpine-treated rats were intravenously transplanted 22 days after SE with BMMCs obtained from enhanced green fluorescent protein (eGFP) transgenic C57BL/6 mice. Control epileptic animals were given an equivalent volume of saline or fibroblast injections. Animals were video-monitored for the presence of spontaneous recurrent seizures prior to and following the cell administration procedure. In addition, rats underwent cognitive evaluation using a Morris water maze.
Key findings: Our data show that BMMCs reduced the frequency of seizures and improved the learning and long-term spatial memory impairments of epileptic rats. EGFP-positive cells were detected in the brains of transplanted animals by PCR analysis.
Significance: The positive behavioral effects observed in our study indicate that BMMCs could represent a promising therapeutic option in the management of chronic temporal lobe epilepsy.
Copyright © 2011 Elsevier Inc. All rights reserved.
Similar articles
- Concise Review: Prospects of Bone Marrow Mononuclear Cells and Mesenchymal Stem Cells for Treating Status Epilepticus and Chronic Epilepsy.
Agadi S, Shetty AK. Agadi S, et al. Stem Cells. 2015 Jul;33(7):2093-103. doi: 10.1002/stem.2029. Epub 2015 May 13. Stem Cells. 2015. PMID: 25851047 Free PMC article. Review. - Behavioral and cognitive alterations, spontaneous seizures, and neuropathology developing after a pilocarpine-induced status epilepticus in C57BL/6 mice.
Müller CJ, Gröticke I, Bankstahl M, Löscher W. Müller CJ, et al. Exp Neurol. 2009 Sep;219(1):284-97. doi: 10.1016/j.expneurol.2009.05.035. Epub 2009 Jun 3. Exp Neurol. 2009. PMID: 19500573 - Prevention of seizures and reorganization of hippocampal functions by transplantation of bone marrow cells in the acute phase of experimental epilepsy.
Costa-Ferro ZS, Vitola AS, Pedroso MF, Cunha FB, Xavier LL, Machado DC, Soares MB, Ribeiro-dos-Santos R, DaCosta JC. Costa-Ferro ZS, et al. Seizure. 2010 Mar;19(2):84-92. doi: 10.1016/j.seizure.2009.12.003. Epub 2010 Jan 18. Seizure. 2010. PMID: 20080419 - Behavioral alterations in the pilocarpine model of temporal lobe epilepsy in mice.
Gröticke I, Hoffmann K, Löscher W. Gröticke I, et al. Exp Neurol. 2007 Oct;207(2):329-49. doi: 10.1016/j.expneurol.2007.06.021. Epub 2007 Jul 12. Exp Neurol. 2007. PMID: 17714705 - Cell therapy in models for temporal lobe epilepsy.
Raedt R, Van Dycke A, Vonck K, Boon P. Raedt R, et al. Seizure. 2007 Oct;16(7):565-78. doi: 10.1016/j.seizure.2007.05.003. Epub 2007 Jun 12. Seizure. 2007. PMID: 17566770 Review.
Cited by
- Early transplantation of bone marrow mononuclear cells promotes neuroprotection and modulation of inflammation after status epilepticus in mice by paracrine mechanisms.
Leal MM, Costa-Ferro ZS, Souza BS, Azevedo CM, Carvalho TM, Kaneto CM, Carvalho RH, Dos Santos RR, Soares MB. Leal MM, et al. Neurochem Res. 2014 Feb;39(2):259-68. doi: 10.1007/s11064-013-1217-7. Epub 2013 Dec 17. Neurochem Res. 2014. PMID: 24343530 - The great migration of bone marrow-derived stem cells toward the ischemic brain: therapeutic implications for stroke and other neurological disorders.
Borlongan CV, Glover LE, Tajiri N, Kaneko Y, Freeman TB. Borlongan CV, et al. Prog Neurobiol. 2011 Oct;95(2):213-28. doi: 10.1016/j.pneurobio.2011.08.005. Epub 2011 Aug 30. Prog Neurobiol. 2011. PMID: 21903148 Free PMC article. Review. - Insight into Ginkgo biloba L. Extract on the Improved Spatial Learning and Memory by Chemogenomics Knowledgebase, Molecular Docking, Molecular Dynamics Simulation, and Bioassay Validations.
Chen Y, Feng Z, Shen M, Lin W, Wang Y, Wang S, Li C, Wang S, Chen M, Shan W, Xie XQ. Chen Y, et al. ACS Omega. 2020 Jan 28;5(5):2428-2439. doi: 10.1021/acsomega.9b03960. eCollection 2020 Feb 11. ACS Omega. 2020. PMID: 32064403 Free PMC article. - Cognitive comorbidities in the rat pilocarpine model of epilepsy.
Guarino A, Pignata P, Lovisari F, Asth L, Simonato M, Soukupova M. Guarino A, et al. Front Neurol. 2024 May 30;15:1392977. doi: 10.3389/fneur.2024.1392977. eCollection 2024. Front Neurol. 2024. PMID: 38872822 Free PMC article. - Concise Review: Prospects of Bone Marrow Mononuclear Cells and Mesenchymal Stem Cells for Treating Status Epilepticus and Chronic Epilepsy.
Agadi S, Shetty AK. Agadi S, et al. Stem Cells. 2015 Jul;33(7):2093-103. doi: 10.1002/stem.2029. Epub 2015 May 13. Stem Cells. 2015. PMID: 25851047 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical