Use of salicylate as a probe for .OH formation in isolated ischemic rat hearts - PubMed (original) (raw)
Use of salicylate as a probe for .OH formation in isolated ischemic rat hearts
S R Powell et al. Free Radic Biol Med. 1990.
Abstract
Salicylic acid was used as a probe for .OH formed during reperfusion of the ischemic myocardium. .OH adds to the phenolic ring of salicylate to yield dihydroxybenzoic acid species. The two principal dihydroxybenzoic acids formed are the 2,3- and 2,5-derivatives and can be isolated and quantitated using HPLC combined with electrochemical detection. In these experiments, dihydroxybenzoic acids were detectable in the f molar range. Rat hearts were perfused in the Langendorff mode with Krebs-Henseleit buffer containing 100 microM salicylate. Following 20 min of global ischemia a 173% increase in tissue content of 2,5-dihydroxybenzoic acid was detected after 2.5 min of reperfusion. The duration of ischemia did not significantly affect tissue content of 2,5-dihydroxybenzoic acid peaked at 250 to 300% of control within 2.5 min of reperfusion. The inclusion of 100 microM salicylate in the perfusion buffer had no effect on myocardial function during the duration of the experiments. The results indicate that salicylate can be used as a very sensitive probe for .OH in the isolated ischemic heart.
Similar articles
- Detection of hydroxyl radicals in the post-ischemic reperfused heart using salicylate as a trapping agent.
Onodera T, Ashraf M. Onodera T, et al. J Mol Cell Cardiol. 1991 Mar;23(3):365-70. doi: 10.1016/0022-2828(91)90072-t. J Mol Cell Cardiol. 1991. PMID: 1652647 - Quantification of hydroxyl radical and its lack of relevance to myocardial injury during early reperfusion after graded ischemia in rat hearts.
Takemura G, Onodera T, Ashraf M. Takemura G, et al. Circ Res. 1992 Jul;71(1):96-105. doi: 10.1161/01.res.71.1.96. Circ Res. 1992. PMID: 1318798 - Salicylate as an in vivo free radical trap: studies on ischemic insult to the rat intestine.
Udassin R, Ariel I, Haskel Y, Kitrossky N, Chevion M. Udassin R, et al. Free Radic Biol Med. 1991;10(1):1-6. doi: 10.1016/0891-5849(91)90014-t. Free Radic Biol Med. 1991. PMID: 1646748 - Salicylate trapping of .OH as a tool for studying post-ischemic oxidative injury in the isolated rat heart.
Powell SR. Powell SR. Free Radic Res. 1994 Nov-Dec;21(6):355-70. doi: 10.3109/10715769409056588. Free Radic Res. 1994. PMID: 7834050 Review. - Hydroxyl free radical (.OH) formation reflected by salicylate hydroxylation and neuromelanin. In vivo markers for oxidant injury of nigral neurons.
Chiueh CC, Murphy DL, Miyake H, Lang K, Tulsi PK, Huang SJ. Chiueh CC, et al. Ann N Y Acad Sci. 1993 May 28;679:370-5. doi: 10.1111/j.1749-6632.1993.tb18324.x. Ann N Y Acad Sci. 1993. PMID: 8390147 Review. No abstract available.
Cited by
- The ubiquitin proteasome system and myocardial ischemia.
Calise J, Powell SR. Calise J, et al. Am J Physiol Heart Circ Physiol. 2013 Feb 1;304(3):H337-49. doi: 10.1152/ajpheart.00604.2012. Epub 2012 Dec 7. Am J Physiol Heart Circ Physiol. 2013. PMID: 23220331 Free PMC article. Review. - The ubiquitin-proteasome system and cardiovascular disease.
Powell SR, Herrmann J, Lerman A, Patterson C, Wang X. Powell SR, et al. Prog Mol Biol Transl Sci. 2012;109:295-346. doi: 10.1016/B978-0-12-397863-9.00009-2. Prog Mol Biol Transl Sci. 2012. PMID: 22727426 Free PMC article. Review. - HPLC analysis of tetrahydrobiopterin and its pteridine derivatives using sequential electrochemical and fluorimetric detection: application to tetrahydrobiopterin autoxidation and chemical oxidation.
Biondi R, Ambrosio G, De Pascali F, Tritto I, Capodicasa E, Druhan LJ, Hemann C, Zweier JL. Biondi R, et al. Arch Biochem Biophys. 2012 Apr 1;520(1):7-16. doi: 10.1016/j.abb.2012.01.010. Epub 2012 Jan 20. Arch Biochem Biophys. 2012. PMID: 22286026 Free PMC article. - Nitric oxide and MPP+-induced hydroxyl radical generation.
Obata T. Obata T. J Neural Transm (Vienna). 2006 Sep;113(9):1131-44. doi: 10.1007/s00702-005-0415-0. Epub 2006 Feb 6. J Neural Transm (Vienna). 2006. PMID: 16463115 Review. - Environmental estrogen-like chemicals and hydroxyl radicals induced by MPTP in the striatum: a review.
Obata T. Obata T. Neurochem Res. 2002 May;27(5):423-31. doi: 10.1023/a:1015556015299. Neurochem Res. 2002. PMID: 12064359 Review.