Detection of hepatitis C virus RNA in sera and liver tissues of non-A, non-B hepatitis patients using the polymerase chain reaction - PubMed (original) (raw)
Detection of hepatitis C virus RNA in sera and liver tissues of non-A, non-B hepatitis patients using the polymerase chain reaction
S Ohkoshi et al. Jpn J Cancer Res. 1990 Sep.
Abstract
Sera obtained from patients with non-A, non-B hepatitis were examined for the presence of hepatitis C virus (HCV) genome by using the reverse transcription-polymerase chain reaction assay, as well as for antibody to HCV (anti-HCV) by using an enzyme-linked immunosorbent assay (ELISA). We also examined the presence of HCV RNA in liver tissue obtained by surgical resection of hepatocellular carcinoma. Among 33 patients, HCV RNA was detectable in 21 (64%), and the antibody was also positive in 21 (64%). Eighteen (55%) patients were positive for both assays. The two assays gave inconsistent results in 3 patients who were positive for HCV RNA but negative for anti-HCV, and in 3 other patients who were negative for HCV RNA and positive for anti-HCV. HCV RNA was also detected in 6 out of 10 non-cancerous liver tissue specimens and in 3 out of 7 tumor tissue specimens. Using the polymerase chain reaction, the HCV genome was detected directly in many specimens obtained from patients with non-A, non-B hepatitis, suggesting the presence of replicating virus in patients positive for anti-HCV. In addition, the differing results of the two assay systems suggest that the application of both is important for evaluation of the status of HCV infection.
Similar articles
- Hepatitis C virus infection in hepatocellular carcinoma. Detection of plus-strand and minus-strand viral RNA.
Takeda S, Shibata M, Morishima T, Harada A, Nakao A, Takagi H, Nagai Y. Takeda S, et al. Cancer. 1992 Nov 1;70(9):2255-9. doi: 10.1002/1097-0142(19921101)70:9<2255::aid-cncr2820700907>3.0.co;2-n. Cancer. 1992. PMID: 1327494 - [Serodiagnosis of hepatitis C by a recombinant hepatitis C virus protein].
Hayashi N, Yuki N, Kasahara A, Fusamoto H, Kamada T. Hayashi N, et al. Nihon Rinsho. 1993 Feb;51(2):329-33. Nihon Rinsho. 1993. PMID: 7681883 Review. Japanese. - Hepatitis C viral RNA amplification by the polymerase chain reaction allows detection of false positive HCV ELISAs among patients with non-A, non-B hepatitis.
Rodríguez-Medina JR, Carrasquillo EA, Sarriera JE, Medina R, Oharriz JJ, Rubio CE. Rodríguez-Medina JR, et al. P R Health Sci J. 1993 Apr;12(1):35-8. P R Health Sci J. 1993. PMID: 8390064 - Hepatitis C virus RNA in southern African blacks with hepatocellular carcinoma.
Bukh J, Miller RH, Kew MC, Purcell RH. Bukh J, et al. Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1848-51. doi: 10.1073/pnas.90.5.1848. Proc Natl Acad Sci U S A. 1993. PMID: 8383330 Free PMC article. - [Summary and evaluation of genome and antibody diagnosis in hepatitis C virus infection].
Nishizono A. Nishizono A. Rinsho Byori. 1994 Dec;42(12):1240-7. Rinsho Byori. 1994. PMID: 7532727 Review. Japanese.
Cited by
- A structural protein encoded by the 5' region of the hepatitis C virus genome efficiently detects viral infection.
Kato N, Hijikata M, Ootsuyama Y, Nakagawa M, Ohkoshi S, Shimotohno K. Kato N, et al. Jpn J Cancer Res. 1990 Nov;81(11):1092-4. doi: 10.1111/j.1349-7006.1990.tb02518.x. Jpn J Cancer Res. 1990. PMID: 2176200 Free PMC article. - Clinical evaluation of the antibody against core protein of hepatitis C virus.
Ohkoshi S, Watanabe M, Kuwana K, Tawaraya H, Kamimura T, Asakura H. Ohkoshi S, et al. Gastroenterol Jpn. 1993 May;28 Suppl 5:80-3. doi: 10.1007/BF02989212. Gastroenterol Jpn. 1993. PMID: 7689513 - Importance of primer selection for the detection of hepatitis C virus RNA with the polymerase chain reaction assay.
Bukh J, Purcell RH, Miller RH. Bukh J, et al. Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):187-91. doi: 10.1073/pnas.89.1.187. Proc Natl Acad Sci U S A. 1992. PMID: 1309604 Free PMC article. - Molecular biology made easy. The polymerase chain reaction.
Clarke AM, Mapstone NP, Quirke P. Clarke AM, et al. Histochem J. 1992 Dec;24(12):913-26. doi: 10.1007/BF01046497. Histochem J. 1992. PMID: 1490867 Review. No abstract available. - Hepatic neoplasia: reflections and ruminations.
Aterman K. Aterman K. Virchows Arch. 1995;427(1):1-18. doi: 10.1007/BF00203732. Virchows Arch. 1995. PMID: 7551337 Review. No abstract available.
References
- ) Dienstag , J. L.Non‐A, non‐B hepatitis, I: recognition, epidemiology and clinical features . Gastroenterology , 85 , 439 – 462 ( 1983. ). - PubMed
- ) Choo , Q‐L. , Kuo , G. , Weiner , A. J. , Overby , L. R. , Bradly , D. W. and Houghton , M.Isolation of a cDNA clone derived from a blood‐borne non‐A, non‐B viral hepatitis genome . Science , 244 , 359 – 362 ( 1989. ). - PubMed
- ) Kuo , G. , Choo Q‐L. , Alter , H. J. , Gitnick , J. L. , Redecker , A. G. , Purcell , R. H. , Miyamura , T. , Dienstag , J. L. , Alter , M. J. , Stevens , C. E. , Tegtmeier , G. E. , Bonino , F. , Colombo , M. , Lee , W‐S. , Kuo , C. , Berger , K. , Shuster , J. R. , Overby , L. R. , Bradly , D. W. and Houghton , M.An assay for circulating antibodies to a major etiologic virus of human non‐A, non‐B hepatitis . Science , 244 , 362 – 364 ( 1989. ). - PubMed
- ) Miyamura , T. , Saito , I. , Katayama , T. , Kikuchi , S. , Tateda , A. , Houghton , M. , Choo , Q‐L. and Kuo , G.Detection of antibody against antigen expressed by molecularly cloned hepatitis C virus cDNA: application to diagnosis and blood screening for post‐transfusion hepatitis . Proc. Natl. Acad. Sci. USA , 87 , 983 – 987 ( 1990. ). - PMC - PubMed
- ) Ohkoshi , S. , Kojima , H. , Tawaraya , H. , Miyajima , T. , Kamimura , T. , Asakura , H. , Satoh , A. , Hirose , S. , Hijikata , M. , Kato , N. and Shimotohno , K.Prevalence of antibody against non‐A, non‐B hepatitis virus in Japanese patients with hepatocellular carcinoma . Jpn. J. Cancer Res. , 81 , 550 – 553 ( 1990. ). - PMC - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical