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Neuroblastoma

Nadja C Colon et al. Adv Pediatr. 2011.

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Figure 1

Figure 1

International Neuroblastoma Risk Group (INRG) Classification. L1, localized tumor confined to one body compartment and with absence of image-defined risk factors (IDRFs); L2, locoregional tumor with presence of one or more IDRFs; M, distant metastatic disease (except stage MS); MS, metastatic disease confined to skin, liver and/or bone marrow in children < 18 months of age; EFS, event-free survival (From Cohn et al, The International Neuroblastoma Risk Group (INRG) Classification System: An INRG Task Force Report.J Clin Onc; 2009; Fig. 2, p.295; with permission).

Figure 2

Figure 2

Effects of GRP-receptor knockdown on neuroblastoma metastasis in vivo. Spleen injection liver metastasis murine model was used using human neuroblastoma BE(2)-C cells transfected with pEGFP/shGRP-R (silenced GRP receptors) or pEGFP/shCON (controls). (A) Representative images of livers from mice injected with BE(2)-C/GFP/shGRP-R (short-hairpin silenced GRP-receptors) and control cells (shCON). Silencing of GRP-receptors (shGRP-R; right column) attenuated liver metastasis of BE(2)-C tumors. Quantitative analysis of liver weight relative to body weight (* p<0.05 vs. shCON) shows significantly less weight in GRP-R silenced group (B) Representative H&E-stained sections of livers from mice injected with BE(2)-C/GFP/shCON (controls; upper) and BE(2)-C/GFP/shGRP-R (GRP receptor silenced; lower) cells. Extensive liver metastases are seen in mice from constitutive GRP-receptor expressing BE(2)-C cells (upper). (Adapted from Qiao et al, Proc Natl Acad Sci 2008; Fig. 6, p.12895. Copyright2008 National Academy of Sciences, U.S.A).

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