Fluorescence recovery after photobleaching - PubMed (original) (raw)
Fluorescence recovery after photobleaching
Alex Carisey et al. Methods Mol Biol. 2011.
Abstract
This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as mobile fraction and half-time of recovery.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous