Differential roles of CCN family proteins during osteoblast differentiation: Involvement of Smad and MAPK signaling pathways - PubMed (original) (raw)
doi: 10.1016/j.bone.2011.06.033. Epub 2011 Jul 7.
Satoshi Kubota, Akiko Suzuki, Makoto Suzuki, Kumiko Kohsaka, Kenji Hoshi, Toshiya Fujii, Noureddine Lazar, Toshihiro Ohgawara, Takeyasu Maeda, Bernard Perbal, Teruko Takano-Yamamoto, Masaharu Takigawa
Affiliations
- PMID: 21763478
- DOI: 10.1016/j.bone.2011.06.033
Differential roles of CCN family proteins during osteoblast differentiation: Involvement of Smad and MAPK signaling pathways
Harumi Kawaki et al. Bone. 2011 Nov.
Abstract
CCN family proteins play diverse roles in many aspects of cellular processes such as proliferation, differentiation, adhesion, migration, angiogenesis and survival. In the bone tissue of vertebrate species, the expression of most CCN family members has been observed in osteoblasts. However, their spatial and temporal distributions, as well as their functions, are still only partially understood. In this study, we evaluated the localization of CCN family members in skeletal tissue in vivo and comparatively analyzed the gene expression patterns and functions of the members in murine osteoblasts in primary culture. Immunofluorescent analyses revealed that the CCN family members were differentially produced in osteoblasts and osteocytes. The presence of all Ccn transcripts was confirmed in those osteoblasts. Among the members, CCN1, CCN2, CCN4 and CCN5 were found in osteocytes. CCN4 and CCN5 were distributed in osteocytes located inside of bone matrix as well. Next, we investigated the expression pattern of Ccn family members during osteoblast differentiation. Along with differentiation, most of the members followed proper gene expression patterns; whereas, Ccn4 and Ccn5 showed quite similar patterns. Furthermore, we evaluated the effects of CCN family members on the osteoblastic activities by using recombinant CCN proteins and RNA interference method. Five members of this family displayed positive effects on osteoblast proliferation or differentiation. Of note, CCN3 drastically inhibited the osteoblast activities. Each Ccn specific siRNA could modulate osteoblast activities in a manner expected by the observed effect of respective recombinant CCN protein. In addition, we found that extracellular signal-regulated kinase1/2 and p38 mitogen-activated protein kinase pathways were critically involved in the CCN family member-mediated modification of osteoblast activities. Collectively, all Ccn family members were found to be differentially expressed along with differentiation and therefore could participate in progression of the osteoblast lineage.
Copyright © 2011 Elsevier Inc. All rights reserved.
Similar articles
- PTHrP signaling targets cyclin D1 and induces osteoblastic cell growth arrest.
Datta NS, Chen C, Berry JE, McCauley LK. Datta NS, et al. J Bone Miner Res. 2005 Jun;20(6):1051-64. doi: 10.1359/JBMR.050106. Epub 2005 Jan 18. J Bone Miner Res. 2005. PMID: 15883646 - Osthole-mediated cell differentiation through bone morphogenetic protein-2/p38 and extracellular signal-regulated kinase 1/2 pathway in human osteoblast cells.
Kuo PL, Hsu YL, Chang CH, Chang JK. Kuo PL, et al. J Pharmacol Exp Ther. 2005 Sep;314(3):1290-9. doi: 10.1124/jpet.105.085092. Epub 2005 Jun 13. J Pharmacol Exp Ther. 2005. PMID: 15956019 - Role of CCN, a vertebrate specific gene family, in development.
Katsube K, Sakamoto K, Tamamura Y, Yamaguchi A. Katsube K, et al. Dev Growth Differ. 2009 Jan;51(1):55-67. doi: 10.1111/j.1440-169X.2009.01077.x. Dev Growth Differ. 2009. PMID: 19128405 Review. - The CCN proteins: important signaling mediators in stem cell differentiation and tumorigenesis.
Zuo GW, Kohls CD, He BC, Chen L, Zhang W, Shi Q, Zhang BQ, Kang Q, Luo J, Luo X, Wagner ER, Kim SH, Restegar F, Haydon RC, Deng ZL, Luu HH, He TC, Luo Q. Zuo GW, et al. Histol Histopathol. 2010 Jun;25(6):795-806. doi: 10.14670/HH-25.795. Histol Histopathol. 2010. PMID: 20376786 Free PMC article. Review.
Cited by
- Current status of Er:YAG laser in periodontal surgery.
Aoki A, Mizutani K, Taniguchi Y, Lin T, Ohsugi Y, Mikami R, Katagiri S, Meinzer W, Iwata T. Aoki A, et al. Jpn Dent Sci Rev. 2024 Dec;60:1-14. doi: 10.1016/j.jdsr.2023.11.002. Epub 2023 Dec 9. Jpn Dent Sci Rev. 2024. PMID: 38148873 Free PMC article. Review. - Engineering a Pro-Osteogenic Secretome through the Transient Silencing of the Gene Encoding Secreted Frizzled Related Protein 1.
García-Sánchez D, González-González A, Álvarez-Iglesias I, Dujo-Gutiérrez MD, Bolado-Carrancio A, Certo M, Pérez-Núñez MI, Riancho JA, Rodríguez-Rey JC, Delgado-Calle J, Pérez-Campo FM. García-Sánchez D, et al. Int J Mol Sci. 2023 Aug 3;24(15):12399. doi: 10.3390/ijms241512399. Int J Mol Sci. 2023. PMID: 37569774 Free PMC article. - The regulation and functions of the matricellular CCN proteins induced by shear stress.
Wang YK, Weng HK, Mo FE. Wang YK, et al. J Cell Commun Signal. 2023 Jun;17(2):361-370. doi: 10.1007/s12079-023-00760-z. Epub 2023 May 16. J Cell Commun Signal. 2023. PMID: 37191841 Free PMC article. Review. - Myeloid CCN3 protects against aortic valve calcification.
Tu P, Xu Q, Zhou X, Villa-Roel N, Kumar S, Dong N, Jo H, Ou C, Lin Z. Tu P, et al. Cell Commun Signal. 2023 Jan 20;21(1):14. doi: 10.1186/s12964-022-01020-0. Cell Commun Signal. 2023. PMID: 36670446 Free PMC article. - Molecular and Genetic Interactions between CCN2 and CCN3 behind Their Yin-Yang Collaboration.
Kubota S, Kawata K, Hattori T, Nishida T. Kubota S, et al. Int J Mol Sci. 2022 May 24;23(11):5887. doi: 10.3390/ijms23115887. Int J Mol Sci. 2022. PMID: 35682564 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous