Metabolomics reveals phospholipids as important nutrient sources during Salmonella growth in bile in vitro and in vivo - PubMed (original) (raw)

Metabolomics reveals phospholipids as important nutrient sources during Salmonella growth in bile in vitro and in vivo

L Caetano M Antunes et al. J Bacteriol. 2011 Sep.

Abstract

During the colonization of hosts, bacterial pathogens are presented with many challenges that must be overcome for colonization to occur successfully. This requires the bacterial sensing of the surroundings and adaptation to the conditions encountered. One of the major impediments to the pathogen colonization of the mammalian gastrointestinal tract is the antibacterial action of bile. Salmonella enterica serovar Typhimurium has specific mechanisms involved in resistance to bile. Additionally, Salmonella can successfully multiply in bile, using it as a source of nutrients. This accomplishment is highly relevant to pathogenesis, as Salmonella colonizes the gallbladder of hosts, where it can be carried asymptomatically and promote further host spread and transmission. To gain insights into the mechanisms used by Salmonella to grow in bile, we studied the changes elicited by Salmonella in the chemical composition of bile during growth in vitro and in vivo through a metabolomics approach. Our data suggest that phospholipids are an important source of carbon and energy for Salmonella during growth in the laboratory as well as during gallbladder infections of mice. Further studies in this area will generate a better understanding of how Salmonella exploits this generally hostile environment for its own benefit.

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Figures

Fig. 1.

Salmonella growth curves in minimal medium with various carbon and energy sources. (A) Salmonella was cultured in M9 minimal medium containing 200 mM histidine (His; circles) or 0.1% Casamino Acids (CAA; squares). Cultures without lyso-PC are represented by gray symbols, whereas cultures containing lyso-PC are indicated by black symbols. Results shown represent the averages from three cultures, and bars indicate the standard deviations. (B) Salmonella was grown in M9 minimal medium with 200 mM histidine (His). Cultures with no addition are represented by diamonds. Circles indicate cultures with glucose (Glu; 10 mg/ml), whereas squares indicate cultures with lyso-PC (1 mg/ml). Results shown represent averages from three cultures, and bars indicate the standard deviations.

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Metabolomics reveals phospholipids as important nutrient sources during Salmonella growth in bile in vitro and in vivo - PubMed (original) (raw)