Cyclic AMP-dependent protein kinase decreases GABAA receptor current in mouse spinal neurons - PubMed (original) (raw)
Cyclic AMP-dependent protein kinase decreases GABAA receptor current in mouse spinal neurons
N M Porter et al. Neuron. 1990 Dec.
Free article
Abstract
GABA, the major inhibitory neurotransmitter in the mammalian brain, binds to GABAA receptors, which form chloride ion channels. The predicted structure of the GABAA receptor places a consensus phosphorylation site for cAMP-dependent protein kinase (PKA) on an intracellular domain of the channel. Phosphorylation by various protein kinases has been shown to alter the activity of certain ligand- and voltage-gated ion channels. We have examined the role of phosphorylation by the catalytic subunit of PKA in the regulation of GABAA receptor channel function using whole-cell and excised outside-out patch-clamp techniques. Inclusion of the catalytic subunit of PKA in the recording pipettes significantly reduced GABA-evoked whole-cell and single-channel chloride currents. Both heat inactivation of PKA and addition of the specific protein kinase inhibitor peptide prevented the reduction of GABA-evoked currents by PKA. Neither mean channel open time nor channel conductance was affected by PKA. The reduction in GABA receptor current by PKA was primarily due to a reduction in channel opening frequency.
Similar articles
- Functional modulation of GABAA receptors by cAMP-dependent protein phosphorylation.
Moss SJ, Smart TG, Blackstone CD, Huganir RL. Moss SJ, et al. Science. 1992 Jul 31;257(5070):661-5. doi: 10.1126/science.1323140. Science. 1992. PMID: 1323140 - Protein kinase modulation of GABAA currents in rabbit retinal rod bipolar cells.
Gillette MA, Dacheux RF. Gillette MA, et al. J Neurophysiol. 1996 Nov;76(5):3070-86. doi: 10.1152/jn.1996.76.5.3070. J Neurophysiol. 1996. PMID: 8930256 - Neurosteroid regulation of GABAA receptor single-channel kinetic properties of mouse spinal cord neurons in culture.
Twyman RE, Macdonald RL. Twyman RE, et al. J Physiol. 1992 Oct;456:215-45. doi: 10.1113/jphysiol.1992.sp019334. J Physiol. 1992. PMID: 1338096 Free PMC article. - Electrophysiological characterization of diazepam binding inhibitor (DBI) on GABAA receptors.
Bormann J. Bormann J. Neuropharmacology. 1991 Dec;30(12B):1387-9. doi: 10.1016/s0028-3908(11)80006-7. Neuropharmacology. 1991. PMID: 1723508 Review. - GABAA receptors: ligand-gated Cl- ion channels modulated by multiple drug-binding sites.
Sieghart W. Sieghart W. Trends Pharmacol Sci. 1992 Dec;13(12):446-50. doi: 10.1016/0165-6147(92)90142-s. Trends Pharmacol Sci. 1992. PMID: 1338138 Review.
Cited by
- Contribution of adenylyl cyclase modulation of pre- and postsynaptic GABA neurotransmission to morphine antinociception and tolerance.
Bobeck EN, Chen Q, Morgan MM, Ingram SL. Bobeck EN, et al. Neuropsychopharmacology. 2014 Aug;39(9):2142-52. doi: 10.1038/npp.2014.62. Epub 2014 Mar 13. Neuropsychopharmacology. 2014. PMID: 24622471 Free PMC article. - Substance P suppresses GABAA receptor function via protein kinase C in primary sensory neurones of bullfrogs.
Yamada K, Akasu T. Yamada K, et al. J Physiol. 1996 Oct 15;496 ( Pt 2)(Pt 2):439-49. doi: 10.1113/jphysiol.1996.sp021697. J Physiol. 1996. PMID: 8910228 Free PMC article. - Ca(2+)-induced rebound potentiation of gamma-aminobutyric acid-mediated currents requires activation of Ca2+/calmodulin-dependent kinase II.
Kano M, Kano M, Fukunaga K, Konnerth A. Kano M, et al. Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13351-6. doi: 10.1073/pnas.93.23.13351. Proc Natl Acad Sci U S A. 1996. PMID: 8917594 Free PMC article. - Adenylate Cyclase 1 Links Calcium Signaling to CFTR-Dependent Cytosolic Chloride Elevations in Chick Amacrine Cells.
Zhong L, Gleason EL. Zhong L, et al. Front Cell Neurosci. 2021 Aug 11;15:726605. doi: 10.3389/fncel.2021.726605. eCollection 2021. Front Cell Neurosci. 2021. PMID: 34456687 Free PMC article. - Activation of 5-HT1A Receptors Promotes Retinal Ganglion Cell Function by Inhibiting the cAMP-PKA Pathway to Modulate Presynaptic GABA Release in Chronic Glaucoma.
Zhou X, Zhang R, Zhang S, Wu J, Sun X. Zhou X, et al. J Neurosci. 2019 Feb 20;39(8):1484-1504. doi: 10.1523/JNEUROSCI.1685-18.2018. Epub 2018 Dec 12. J Neurosci. 2019. PMID: 30541912 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources