Predisposition to the development of IL-9-secreting T cells in atopic infants - PubMed (original) (raw)

Comment

Predisposition to the development of IL-9-secreting T cells in atopic infants

Weiguo Yao et al. J Allergy Clin Immunol. 2011 Dec.

No abstract available

PubMed Disclaimer

Figures

Fig. 1

Fig. 1

IL-9 concentration in anti-CD3- or allergen-stimulated PBMC from atopic and non-atopic patients. A. PBMCs were stimulated with anti-CD3 for 72 hours before supernatants were assayed for IL-9 concentration using ELISA. Samples were divided into non-atopic patients (n=34), or patients that had allergen-specific IgE for egg (n=20), cat (n=29) or house dust mite (n=28). B–C, IL-9 (B) or Th2 cytokine (C) concentration was determined in supernatants of PBMC stimulated for seven days of culture with cat dander extract- or house dust mite extract (10 μg/ml) using cells isolated from atopic (n=5–10, black bars) or non-atopic (n=10–11, white bars) patients. *:p<0.05 by Student’s t test.

Fig. 2

Fig. 2

In vitro Th9 differentiation of naïve (CD4+ CD45RA+) T cells from atopic and non-atopic patients. A. IL9, PU.1 (SPI1), and IRF4 gene expression in Th9 cells derived from naïve CD4+ T cells isolated from non-atopic (n=11) and atopic patients (n=10) and cultured for five days with TGFβ and IL-4. B. IL-9 production from Th9 cells derived as in (A). C. The percentage of pSTAT6-positive cells was assessed at day 4 of differentiation under Th9 conditions in cultures from atopic and non-atopic patients (n=6). *:p<0.05 by Student’s t test.

Comment on

Similar articles

Cited by

References

    1. Goswami R, Kaplan MH. A Brief History of IL-9. J Immunol. 2011;186:3283–8. - PMC - PubMed
    1. Devos S, Cormont F, Vrtala S, Hooghe-Peters E, Pirson F, Snick J. Allergen-induced interleukin-9 production in vitro: correlation with atopy in human adults and comparison with interleukin-5 and interleukin-13. Clin Exp Allergy. 2006;36:174–82. - PubMed
    1. Erpenbeck VJ, Hohlfeld JM, Discher M, Krentel H, Hagenberg A, Braun A, et al. Increased expression of interleukin-9 messenger RNA after segmental allergen challenge in allergic asthmatics. Chest. 2003;123:370S. - PubMed
    1. Jenmalm MC, Van Snick J, Cormont F, Salman B. Allergen-induced Th1 and Th2 cytokine secretion in relation to specific allergen sensitization and atopic symptoms in children. Clin Exp Allergy. 2001;31:1528–35. - PubMed
    1. Tepper RS, Llapur CJ, Jones MH, Tiller C, Coates C, Kimmel R, et al. Expired nitric oxide and airway reactivity in infants at risk for asthma. J Allergy Clin Immunol. 2008;122:760–5. - PMC - PubMed

Publication types

MeSH terms

Substances

Grants and funding

LinkOut - more resources