Evaluation of protein expression in bovine bronchoalveolar fluid following challenge with Mannheimia haemolytica - PubMed (original) (raw)

Comparative Study

. 2011 Sep;11(18):3685-97.

doi: 10.1002/pmic.201000710. Epub 2011 Aug 23.

Affiliations

• PMID: 21800424
• DOI: 10.1002/pmic.201000710

Comparative Study

Evaluation of protein expression in bovine bronchoalveolar fluid following challenge with Mannheimia haemolytica

Jamie L Boehmer et al. Proteomics. 2011 Sep.

Abstract

Proteomics analysis of bovine bronchoalveolar fluid (BAF) following induction of pneumonia with Mannheimia haemolytica using nanoflow liquid chromatography coupled with tandem mass spectrometry (nanoLC-MS/MS) resulted in the identification of 88 unique proteins. Proteins detected in BAF included antimicrobial peptides (AMPs), complement factors, acute-phase proteins, protease inhibitors, and proteins involved in oxidation-reduction. Notwithstanding biological variation, differences in relative protein abundance, determined using normalized peptide counts, were detected for select proteins in BAF from genuinely infected versus sham-infected animals. To demonstrate the applicability of using normalized peptide counts to assess protein expression trends, LC-MS/MS data for the acute-phase protein haptoglobin (HPT) were compared with ELISA data, and statistical evaluation of the relationship between the data revealed a strong measure of association. Differences were detected between sham- and genuinely infected animals for haptoglobin, as well as the AMPs cathelicidin-1 and cathelicidin-4, and inter-α-trypsin inhibitor heavy chain-4, a fairly novel protein involved in the acute phase response. Though the small sample size limited the scope of the inferences, the results indicate the likely importance of AMPs and acute-phase proteins during respiratory infection, and provide additional information regarding potential mechanisms involved in the bovine mucosal barrier defense.

Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PubMed Disclaimer

Similar articles

• Serum concentrations of haptoglobin and haptoglobin-matrix metalloproteinase 9 (Hp-MMP 9) complexes of bovine calves in a bacterial respiratory challenge model.
  Hanthorn CJ, Dewell GA, Dewell RD, Cooper VL, Wang C, Plummer PJ, Lakritz J. Hanthorn CJ, et al. BMC Vet Res. 2014 Dec 6;10:285. doi: 10.1186/s12917-014-0285-5. BMC Vet Res. 2014. PMID: 25480520 Free PMC article.
• Effects of melengestrol acetate on the inflammatory response in heifers challenged with Mannheimia haemolytica.
  Corrigan ME, Drouillard JS, Spire MF, Mosier DA, Minton JE, Higgins JJ, Loe ER, Depenbusch BE, Fox JT. Corrigan ME, et al. J Anim Sci. 2007 Jul;85(7):1770-9. doi: 10.2527/jas.2006-396. Epub 2007 Mar 19. J Anim Sci. 2007. PMID: 17371793
• The proteomic advantage: label-free quantification of proteins expressed in bovine milk during experimentally induced coliform mastitis.
  Boehmer JL, DeGrasse JA, McFarland MA, Tall EA, Shefcheck KJ, Ward JL, Bannerman DD. Boehmer JL, et al. Vet Immunol Immunopathol. 2010 Dec 15;138(4):252-66. doi: 10.1016/j.vetimm.2010.10.004. Epub 2010 Oct 14. Vet Immunol Immunopathol. 2010. PMID: 21067814 Review.
• [Mannheimia haemolytica and the pathogenesis of enzootic bronchopneumonia].
  Ewers C, Lübke-Becker A, Wieler LH. Ewers C, et al. Berl Munch Tierarztl Wochenschr. 2004 Mar-Apr;117(3-4):97-115. Berl Munch Tierarztl Wochenschr. 2004. PMID: 15046457 Review. German.

Cited by

• Purification and characterization of neurotoxin complex from a dual toxin gene containing Clostridium Botulinum Strain PS-5.
  Singh AK, Sachdeva A, Degrasse JA, Croley TR, Stanker LH, Hodge D, Sharma SK. Singh AK, et al. Protein J. 2013 Apr;32(4):288-96. doi: 10.1007/s10930-013-9486-1. Protein J. 2013. PMID: 23625059
• Acute phase proteins as local biomarkers of respiratory infection in calves.
  Prohl A, Schroedl W, Rhode H, Reinhold P. Prohl A, et al. BMC Vet Res. 2015 Jul 25;11:167. doi: 10.1186/s12917-015-0485-7. BMC Vet Res. 2015. PMID: 26209015 Free PMC article.
• A single-stranded DNA aptamer that selectively binds to Staphylococcus aureus enterotoxin B.
  DeGrasse JA. DeGrasse JA. PLoS One. 2012;7(3):e33410. doi: 10.1371/journal.pone.0033410. Epub 2012 Mar 16. PLoS One. 2012. PMID: 22438927 Free PMC article.
• Plasma protein levels of young healthy pigs as indicators of disease resilience.
  Chen Y, Lonergan S, Lim KS, Cheng J, Putz AM, Dyck MK, Canada P, Fortin F, Harding JCS, Plastow GS, Dekkers JCM. Chen Y, et al. J Anim Sci. 2023 Jan 3;101:skad014. doi: 10.1093/jas/skad014. J Anim Sci. 2023. PMID: 36638126 Free PMC article.
• Application of Functional Genomics for Bovine Respiratory Disease Diagnostics.
  Rai AN, Epperson WB, Nanduri B. Rai AN, et al. Bioinform Biol Insights. 2015 Oct 22;9(Suppl 2):13-23. doi: 10.4137/BBI.S30525. eCollection 2015. Bioinform Biol Insights. 2015. PMID: 26526746 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley