DNA damage induction in human cells exposed to vanadium oxides in vitro - PubMed (original) (raw)
Comparative Study
. 2011 Dec;25(8):1996-2002.
doi: 10.1016/j.tiv.2011.07.009. Epub 2011 Jul 22.
Affiliations
- PMID: 21803147
- DOI: 10.1016/j.tiv.2011.07.009
Comparative Study
DNA damage induction in human cells exposed to vanadium oxides in vitro
Juan J Rodríguez-Mercado et al. Toxicol In Vitro. 2011 Dec.
Abstract
Vanadium and vanadium salts cause genotoxicity and elicit variable biological effects depending on several factors. In the present study, we analyzed and compared the DNA damage and repair processes induced by vanadium in three oxidation states. We used human blood leukocytes in vitro and in a single cell gel electrophoresis assay at two pH values. We observed that vanadium(III) trioxide and vanadium(V) pentoxide produced DNA single-strand breaks at all of the concentrations (1, 2, 4, or 8 μg/ml) and treatment times (2, 4, or 6 h) tested. Vanadium(IV) tetraoxide treatment significantly increased DNA damage at all concentrations for 4 or 6 h of treatment but not for 2 h of treatment. The DNA repair kinetics indicated that most of the cells exposed to vanadium III and V for 4 h recovered within the repair incubation time of 90 min; however, those exposed to vanadium(IV) repaired their DNA within 120 min. The data at pH 9 indicated that vanadium(IV) tetraoxide induced DNA double-strand breaks. Our results show that the genotoxic effect of vanadium can be produced by any of its three oxidation states. However, vanadium(IV) induces double-strand breaks, and it is known that these lesions are linked with forming structural chromosomal aberrations.
Copyright © 2011 Elsevier Ltd. All rights reserved.
Similar articles
- Vanadyl sulfate can differentially damage DNA in human lymphocytes and HeLa cells.
Wozniak K, Blasiak J. Wozniak K, et al. Arch Toxicol. 2004 Jan;78(1):7-15. doi: 10.1007/s00204-003-0506-3. Epub 2003 Sep 10. Arch Toxicol. 2004. PMID: 13680095 - Chromosomal damage induced by vanadium oxides in human peripheral lymphocytes.
Rodríguez-Mercado JJ, Alvarez-Barrera L, Altamirano-Lozano MA. Rodríguez-Mercado JJ, et al. Drug Chem Toxicol. 2010;33(1):97-102. doi: 10.3109/01480540903176602. Drug Chem Toxicol. 2010. PMID: 20001663 - Genotoxic effects of vanadium pentoxide on human peripheral lymphocytes and mucosal cells of the upper aerodigestive tract.
Kleinsasser N, Dirschedl P, Staudenmaier R, Harréus U, Wallner B. Kleinsasser N, et al. Int J Environ Health Res. 2003 Dec;13(4):373-9. doi: 10.1080/0960312031000122460. Int J Environ Health Res. 2003. PMID: 14594703 - Genotoxicity of environmental agents assessed by the alkaline comet assay.
Møller P. Møller P. Basic Clin Pharmacol Toxicol. 2005;96 Suppl 1:1-42. Basic Clin Pharmacol Toxicol. 2005. PMID: 15859009 Review. - Vanadium carcinogenic, immunotoxic and neurotoxic effects: a review of in vitro studies.
Zwolak I. Zwolak I. Toxicol Mech Methods. 2014 Jan;24(1):1-12. doi: 10.3109/15376516.2013.843110. Toxicol Mech Methods. 2014. PMID: 24147425 Review.
Cited by
- Risk assessment of rare earth elements, antimony, barium, boron, lithium, tellurium, thallium and vanadium in teas.
Kowalczyk E, Givelet L, Amlund H, Sloth JJ, Hansen M. Kowalczyk E, et al. EFSA J. 2022 May 25;20(Suppl 1):e200410. doi: 10.2903/j.efsa.2022.e200410. eCollection 2022 May. EFSA J. 2022. PMID: 35634564 Free PMC article. - Biological Consequences of Vanadium Effects on Formation of Reactive Oxygen Species and Lipid Peroxidation.
Aureliano M, De Sousa-Coelho AL, Dolan CC, Roess DA, Crans DC. Aureliano M, et al. Int J Mol Sci. 2023 Mar 11;24(6):5382. doi: 10.3390/ijms24065382. Int J Mol Sci. 2023. PMID: 36982458 Free PMC article. Review. - Vanadium Pentoxide Exposure Causes Strain-Dependent Changes in Mitochondrial DNA Heteroplasmy, Copy Number, and Lesions, but Not Nuclear DNA Lesions.
Dobson NL, Kleeberger SR, Burkholder AB, Walters DM, Gladwell W, Gerrish K, Vellers HL. Dobson NL, et al. Int J Mol Sci. 2023 Sep 25;24(19):14507. doi: 10.3390/ijms241914507. Int J Mol Sci. 2023. PMID: 37833956 Free PMC article. - Thallium-induced DNA damage, genetic, and epigenetic alterations.
Sánchez-Chapul L, Santamaría A, Aschner M, Ke T, Tinkov AA, Túnez I, Osorio-Rico L, Galván-Arzate S, Rangel-López E. Sánchez-Chapul L, et al. Front Genet. 2023 Apr 20;14:1168713. doi: 10.3389/fgene.2023.1168713. eCollection 2023. Front Genet. 2023. PMID: 37152998 Free PMC article. Review. - A novel analytical potential function for dicationic diatomic molecular systems based on deformed exponential function.
Machado DFS, Silva RAL, de Oliveira AP, Carvalho-Silva VH, Gargano R, Ribeiro L, de Oliveira HCB. Machado DFS, et al. J Mol Model. 2017 Jun;23(6):182. doi: 10.1007/s00894-017-3339-3. Epub 2017 May 9. J Mol Model. 2017. PMID: 28488189