The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene - PubMed (original) (raw)

The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene

M J Klemsz et al. Cell. 1990.

Abstract

We have isolated a cDNA clone, PU.1, that codes for a new tissue-specific DNA binding protein. Analysis of the binding site by methylation interference and DNAase 1 protection revealed that the PU.1 protein recognized a purine-rich sequence, 5'-GAGGAA-3' (PU box). The PU.1 protein was shown to be a transcriptional activator that is expressed in macrophages and B cells. cDNA constructions used to generate proteins lacking portions of either the amino- or carboxy-terminal ends of the PU.1 protein placed the DNA binding domain in the highly basic carboxy-terminal domain of the protein. The amino acid sequence in the binding domain of PU.1 has considerable identity with proteins belonging to the ets oncogene family.

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Comment in

• The PU.1 transcription factor is the product of the putative oncogene Spi-1.
  Goebl MK. Goebl MK. Cell. 1990 Jun 29;61(7):1165-6. doi: 10.1016/0092-8674(90)90676-6. Cell. 1990. PMID: 2364426 No abstract available.

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