Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition - PubMed (original) (raw)
. 2011 Aug 5;146(3):471-84.
doi: 10.1016/j.cell.2011.06.025.
Wiebke Stahlschmidt, Haydar Bulut, Kira Gromova, Dmytro Puchkov, Mark J Robertson, Kylie A MacGregor, Nikolay Tomilin, Arndt Pechstein, Ngoc Chau, Megan Chircop, Jennette Sakoff, Jens Peter von Kries, Wolfram Saenger, Hans-Georg Kräusslich, Oleg Shupliakov, Phillip J Robinson, Adam McCluskey, Volker Haucke
Affiliations
- PMID: 21816279
- DOI: 10.1016/j.cell.2011.06.025
Free article
Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition
Lisa von Kleist et al. Cell. 2011.
Free article
Erratum in
- Cell. 2011 Sep 2;146(5):841. Tomlin, Nikolay [corrected to Tomilin, Nikolay]
Abstract
Clathrin-mediated endocytosis (CME) regulates many cell physiological processes such as the internalization of growth factors and receptors, entry of pathogens, and synaptic transmission. Within the endocytic network, clathrin functions as a central organizing platform for coated pit assembly and dissociation via its terminal domain (TD). We report the design and synthesis of two compounds named pitstops that selectively block endocytic ligand association with the clathrin TD as confirmed by X-ray crystallography. Pitstop-induced inhibition of clathrin TD function acutely interferes with receptor-mediated endocytosis, entry of HIV, and synaptic vesicle recycling. Endocytosis inhibition is caused by a dramatic increase in the lifetimes of clathrin coat components, including FCHo, clathrin, and dynamin, suggesting that the clathrin TD regulates coated pit dynamics. Pitstops provide new tools to address clathrin function in cell physiology with potential applications as inhibitors of virus and pathogen entry and as modulators of cell signaling.
Copyright © 2011 Elsevier Inc. All rights reserved.
Similar articles
- N-WASP deficiency impairs EGF internalization and actin assembly at clathrin-coated pits.
Benesch S, Polo S, Lai FP, Anderson KI, Stradal TE, Wehland J, Rottner K. Benesch S, et al. J Cell Sci. 2005 Jul 15;118(Pt 14):3103-15. doi: 10.1242/jcs.02444. Epub 2005 Jun 28. J Cell Sci. 2005. PMID: 15985465 - Cargo recognition during clathrin-mediated endocytosis: a team effort.
Sorkin A. Sorkin A. Curr Opin Cell Biol. 2004 Aug;16(4):392-9. doi: 10.1016/j.ceb.2004.06.001. Curr Opin Cell Biol. 2004. PMID: 15261671 Review. - Cargo regulates clathrin-coated pit dynamics.
Puthenveedu MA, von Zastrow M. Puthenveedu MA, et al. Cell. 2006 Oct 6;127(1):113-24. doi: 10.1016/j.cell.2006.08.035. Cell. 2006. PMID: 17018281 - Understanding living clathrin-coated pits.
Rappoport JZ, Simon SM, Benmerah A. Rappoport JZ, et al. Traffic. 2004 May;5(5):327-37. doi: 10.1111/j.1398-9219.2004.00187.x. Traffic. 2004. PMID: 15086782 Review.
Cited by
- Inhibition of clathrin by pitstop 2 activates the spindle assembly checkpoint and induces cell death in dividing HeLa cancer cells.
Smith CM, Haucke V, McCluskey A, Robinson PJ, Chircop M. Smith CM, et al. Mol Cancer. 2013 Jan 17;12:4. doi: 10.1186/1476-4598-12-4. Mol Cancer. 2013. PMID: 23327284 Free PMC article. - Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells.
Chiang CF, Flint M, Lin JS, Spiropoulou CF. Chiang CF, et al. PLoS One. 2016 Oct 25;11(10):e0164768. doi: 10.1371/journal.pone.0164768. eCollection 2016. PLoS One. 2016. PMID: 27780263 Free PMC article. - Neutralization of Virus Infectivity by Antibodies: Old Problems in New Perspectives.
Klasse PJ. Klasse PJ. Adv Biol. 2014;2014:157895. doi: 10.1155/2014/157895. Epub 2014 Sep 9. Adv Biol. 2014. PMID: 27099867 Free PMC article. - Antigenic properties of the human immunodeficiency virus envelope glycoprotein gp120 on virions bound to target cells.
Mengistu M, Ray K, Lewis GK, DeVico AL. Mengistu M, et al. PLoS Pathog. 2015 Mar 25;11(3):e1004772. doi: 10.1371/journal.ppat.1004772. eCollection 2015 Mar. PLoS Pathog. 2015. PMID: 25807494 Free PMC article. - Kir3 channels undergo arrestin-dependant internalization following delta opioid receptor activation.
Nagi K, Charfi I, Pineyro G. Nagi K, et al. Cell Mol Life Sci. 2015 Sep;72(18):3543-57. doi: 10.1007/s00018-015-1899-x. Epub 2015 Apr 22. Cell Mol Life Sci. 2015. PMID: 25900661 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous