Independent localization of MAP2, CaMKIIα and β-actin RNAs in low copy numbers - PubMed (original) (raw)

Figure 4

Neuronal mRNAs are differentially affected by Staufen 2. (A) Neuronal mRNAs were detected in wild-type (untransfected) or Stau2-deficient hippocampal neurons (transfected with shStau2 and coexpressing EGFP, marked with asterisks). Quantification of average cell body signal intensity of MAP2, β-actin, α-tubulin, β-tubulin, GAPDH, septin 7 and CaMKIIα RNAs (B) of neurons transfected with shStau2 or misStau2. For each respective condition, 31, 25, 26, 16, 16, 20, 9, 12, 18, 14, 16, 25, 12 or 18 cells were analysed (in the order they appear on the graph). (C) Numbers of dendritic particles containing MAP2, β-actin, septin 7 and CaMKIIα and (D) average fluorescence intensity of these dendritic particles in shStau2-transfected neurons compared with mismatch control. In panel C, 34, 106, 33, 42, 28, 26, 29 or 25 dendrites, and in panel D, 217, 436, 62, 115, 124, 211, 92 or 139 particles were analysed for each respective condition (in the order they appear on the graphs). Error bars are the s.e.m. of cell body intensities of individual cells (B), detected particles per dendrite (C) or intensities of individual particles (D). **P<0.01 and ***P<0.001. EGFP, enhanced green fluorescent protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; misStau2, mismatch shRNA against Stau2; NS, not significant; shStau2, short hairpin Staufen 2.