Correlation of SATB1 overexpression with the progression of human rectal cancer - PubMed (original) (raw)

Correlation of SATB1 overexpression with the progression of human rectal cancer

Wen-Jian Meng et al. Int J Colorectal Dis. 2012 Feb.

Abstract

Background and aims: To date, the association between special AT-rich sequence-binding protein 1 (SATB1) and colorectal cancer (CRC) has not been reported. This study was aimed at investigating the expression and potential role of SATB1 in human rectal cancers.

Methods: Ninety-three paired samples of rectal cancer and distant normal rectal tissue were analyzed by quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC), and the correlations between SATB1 expression and clinicopathological parameters were evaluated. The expression profiles of SATB1 were also investigated in a panel of five human colon carcinoma cell lines.

Results: The general level of SATB1 mRNA in rectal cancer tissues was statistically significantly higher than that in normal mucosa (P = 0.043). The rate of positive SATB1 protein expression in rectal cancers (44.1%) was significantly higher than that in normal tissues (25.8%) by IHC analysis (P = 0.009). Overexpression of SATB1 mRNA was more predominant in patients with earlier onset of rectal cancer (P = 0.033). SATB1 expression correlated with invasive depth and tumor node metastasis (TNM) stage at both protein and mRNA levels (P < 0.05). Furthermore, SATB1 expression in the poorly metastatic KM12C cells was significantly lower than the highly metastatic KM12SM and KM12L4A cells and higher than the HCT116 and SW480 cells (P = 0.001). These results were further confirmed by Western blotting.

Conclusion: Our results indicate that SATB1 may play an important role in the progression of human rectal cancer, which represents a possible new mechanism underlying CRC.

PubMed Disclaimer

References

    1. APMIS. 2010 Nov;118(11):855-63 - PubMed
    1. Genes Dev. 2000 Mar 1;14(5):521-35 - PubMed
    1. Oncol Rep. 2010 Oct;24(4):981-7 - PubMed
    1. Mol Cell Biol. 2009 Mar;29(5):1321-37 - PubMed
    1. Nature. 2008 Mar 13;452(7184):187-93 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources