Glucose uptake in human and animal muscle cells in culture - PubMed (original) (raw)
Glucose uptake in human and animal muscle cells in culture
V Sarabia et al. Biochem Cell Biol. 1990 Feb.
Abstract
Human muscle cells were grown in culture from satellite cells present in muscle biopsies and fusion-competent clones were identified. Hexose uptake was studied in fused myotubes of human muscle cells in culture and compared with hexose uptake in myotubes of the rat L6 and mouse C2C12 muscle cell lines. Uptake of 2-deoxyglucose was saturable and showed an apparent Km of about 1.5 mM in myotubes of all three cell types. The Vmax of uptake was about 6000 pmol/(min.mg protein) in human cells, 4000 pmol/(min.mg protein) in mouse C2C12 muscle cells, and 500 pmol/(min.mg protein) in L6 cells. Hexose uptake was inhibited approximately 90% by cytochalasin B in human, rat, and mouse muscle cell cultures. Insulin stimulated 2-deoxyglucose uptake in all three cultures. The hormone also stimulated transport of 3-O-methylglucose. The sensitivity to insulin was higher in human and C2C12 mouse myotubes (half-maximal stimulation observed at 3.5 X 10(-9) M) than in rat L6 myotubes (half-maximal stimulation observed at 2.5 X 10(-8) M). However, insulin (10(-6) M) stimulated hexose uptake to a larger extent (2.37-fold) in L6 than in either human (1.58-fold) or mouse (1.39-fold) myotubes. It is concluded that human muscle cells grown in culture display carrier-mediated glucose uptake, with qualitatively similar characteristics to those of other muscle cells, and that insulin stimulates hexose uptake in human cells. These cultures will be instrumental in the study of human insulin resistance and in investigations on the mechanism of action of antidiabetic drugs.
Similar articles
- Stimulation of hexose transport by metformin in L6 muscle cells in culture.
Klip A, Gumà A, Ramlal T, Bilan PJ, Lam L, Leiter LA. Klip A, et al. Endocrinology. 1992 May;130(5):2535-44. doi: 10.1210/endo.130.5.1572281. Endocrinology. 1992. PMID: 1572281 - Hexose transport in L6 muscle cells. Kinetic properties and the number of [3H]cytochalasin B binding sites.
Klip A, Logan WJ, Li G. Klip A, et al. Biochim Biophys Acta. 1982 May 7;687(2):265-80. doi: 10.1016/0005-2736(82)90555-7. Biochim Biophys Acta. 1982. PMID: 7093257 - Effect of endotoxin-induced monokines on glucose metabolism in the muscle cell line L6.
Lee MD, Zentella A, Vine W, Pekala PH, Cerami A. Lee MD, et al. Proc Natl Acad Sci U S A. 1987 May;84(9):2590-4. doi: 10.1073/pnas.84.9.2590. Proc Natl Acad Sci U S A. 1987. PMID: 3472226 Free PMC article. - Induction of sugar uptake response to insulin by serum depletion in fusing L6 myoblasts.
Klip A, Li G, Logan WJ. Klip A, et al. Am J Physiol. 1984 Sep;247(3 Pt 1):E291-6. doi: 10.1152/ajpendo.1984.247.3.E291. Am J Physiol. 1984. PMID: 6383069 - Modulation of hexose transport in cultured skeletal muscle.
Shanahan MF. Shanahan MF. Mol Cell Endocrinol. 1984 Dec;38(2-3):171-8. doi: 10.1016/0303-7207(84)90115-1. Mol Cell Endocrinol. 1984. PMID: 6391979
Cited by
- Rare Sugar Metabolism and Impact on Insulin Sensitivity along the Gut-Liver-Muscle Axis In Vitro.
van Laar A, Grootaert C, Rajkovic A, Desmet T, Beerens K, Van Camp J. van Laar A, et al. Nutrients. 2023 Mar 25;15(7):1593. doi: 10.3390/nu15071593. Nutrients. 2023. PMID: 37049441 Free PMC article. - The dose-response effects of arachidonic acid on primary human skeletal myoblasts and myotubes.
Roberts BM, Kolb AL, Geddis AV, Naimo MA, Matheny RW. Roberts BM, et al. J Int Soc Sports Nutr. 2023 Jan 3;20(1):2164209. doi: 10.1080/15502783.2022.2164209. eCollection 2023. J Int Soc Sports Nutr. 2023. PMID: 36620755 Free PMC article. - On the causal relationships between hyperinsulinaemia, insulin resistance, obesity and dysglycaemia in type 2 diabetes.
Johnson JD. Johnson JD. Diabetologia. 2021 Oct;64(10):2138-2146. doi: 10.1007/s00125-021-05505-4. Epub 2021 Jul 22. Diabetologia. 2021. PMID: 34296322 - The effect of a chemotherapy drug cocktail on myotube morphology, myofibrillar protein abundance, and substrate availability.
Mora S, Adegoke OAJ. Mora S, et al. Physiol Rep. 2021 Jul;9(13):e14927. doi: 10.14814/phy2.14927. Physiol Rep. 2021. PMID: 34197700 Free PMC article. - Glucose Uptake and Insulin Response in Tissue-engineered Human Skeletal Muscle.
Kondash ME, Ananthakumar A, Khodabukus A, Bursac N, Truskey GA. Kondash ME, et al. Tissue Eng Regen Med. 2020 Dec;17(6):801-813. doi: 10.1007/s13770-020-00242-y. Epub 2020 Mar 21. Tissue Eng Regen Med. 2020. PMID: 32200516 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources