Structural and functional characterization of S-adenosylmethionine (SAM) synthetase from Pichia ciferrii - PubMed (original) (raw)

. 2012 Jan;35(1-2):173-81.

doi: 10.1007/s00449-011-0640-x. Epub 2011 Oct 12.

Affiliations

• PMID: 21989639
• DOI: 10.1007/s00449-011-0640-x

Structural and functional characterization of S-adenosylmethionine (SAM) synthetase from Pichia ciferrii

Sangyoung Yoon et al. Bioprocess Biosyst Eng. 2012 Jan.

Abstract

S-adenosylmethionine synthetase (SAM-s) catalyzes the synthesis of S-adenosylmethionine (SAM), which is essential for methylation, transcription, proliferation, and production of secondary metabolites. Here SAM-s from Pichia ciferrii were selectively cloned using RNA CapFishing and rapid amplification of cDNA ends (RACE). The putative full-length cDNA of SAM-s encoded a 383 amino acid protein (42.6 kDa), which has highly conserved metal binding sites, a phosphate-binding site, and functionally important motifs. The corresponding enzyme was over-expressed in a heterologous host of Pichia pastoris, and then purified to a homogenous form. Enzyme kinetics, immunoblotting, circular dichroism (CD), high performance liquid chromatography (HPLC), and molecular modeling were conducted to characterize the SAM-s from P. ciferrii. Structural and functional studies of SAM-s will provide important insights for industrial applications.

PubMed Disclaimer

Similar articles

• DNA shuffling of methionine adenosyltransferase gene leads to improved S-adenosyl-L-methionine production in Pichia pastoris.
  Hu H, Qian J, Chu J, Wang Y, Zhuang Y, Zhang S. Hu H, et al. J Biotechnol. 2009 May 20;141(3-4):97-103. doi: 10.1016/j.jbiotec.2009.03.006. Epub 2009 Mar 27. J Biotechnol. 2009. PMID: 19433211
• Crystal structure of the S-adenosylmethionine synthetase ternary complex: a novel catalytic mechanism of S-adenosylmethionine synthesis from ATP and Met.
  Komoto J, Yamada T, Takata Y, Markham GD, Takusagawa F. Komoto J, et al. Biochemistry. 2004 Feb 24;43(7):1821-31. doi: 10.1021/bi035611t. Biochemistry. 2004. PMID: 14967023
• A genetic method to enhance the accumulation of S-adenosylmethionine in yeast.
  Kanai M, Mizunuma M, Fujii T, Iefuji H. Kanai M, et al. Appl Microbiol Biotechnol. 2017 Feb;101(4):1351-1357. doi: 10.1007/s00253-017-8098-7. Epub 2017 Jan 11. Appl Microbiol Biotechnol. 2017. PMID: 28078396 Review.
• Progress in the research of S-adenosyl-L-methionine production.
  Chu J, Qian J, Zhuang Y, Zhang S, Li Y. Chu J, et al. Appl Microbiol Biotechnol. 2013 Jan;97(1):41-9. doi: 10.1007/s00253-012-4536-8. Epub 2012 Nov 8. Appl Microbiol Biotechnol. 2013. PMID: 23135229 Review.

Cited by

• Overexpression of S-Adenosylmethionine Synthetase in Recombinant Chlamydomonas for Enhanced Lipid Production.
  Kim JH, Ahn JW, Park EJ, Choi JI. Kim JH, et al. J Microbiol Biotechnol. 2023 Mar 28;33(3):310-318. doi: 10.4014/jmb.2212.12009. Epub 2023 Jan 16. J Microbiol Biotechnol. 2023. PMID: 36734134 Free PMC article.
• Proteomic Analysis of Silk Viability in Maize Inbred Lines and Their Corresponding Hybrids.
  Ma Z, Qin Y, Wang Y, Zhao X, Zhang F, Tang J, Fu Z. Ma Z, et al. PLoS One. 2015 Dec 2;10(12):e0144050. doi: 10.1371/journal.pone.0144050. eCollection 2015. PLoS One. 2015. PMID: 26630375 Free PMC article.
• Molecular cloning and characterization of S-adenosylmethionine synthetase gene from Lycoris radiata.
  Li XD, Xia B, Wang R, Xu S, Jiang YM, Yu FB, Peng F. Li XD, et al. Mol Biol Rep. 2013 Feb;40(2):1255-63. doi: 10.1007/s11033-012-2168-9. Epub 2012 Oct 18. Mol Biol Rep. 2013. PMID: 23073776

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Springer