Direct detection of Staphylococcus osteoarticular infections by use of Xpert MRSA/SA SSTI real-time PCR - PubMed (original) (raw)
Direct detection of Staphylococcus osteoarticular infections by use of Xpert MRSA/SA SSTI real-time PCR
Anne Dubouix-Bourandy et al. J Clin Microbiol. 2011 Dec.
Abstract
We evaluated the Xpert MRSA/SA SSTI real-time PCR assay (Cepheid, Sunnyvale, CA) directly on perioperative bone and joint samples. The sensitivity and specificity for detection of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-resistant coagulase-negative Staphylococcus were, respectively, 100% and 98.3%, 100% and 100%, and 100% and 95.3%. The median total test turnaround time was 72 min for PCR versus 79 h for culture. Using these rapid results, appropriate antibiotic treatment could be rapidly initiated.
Figures
Fig. 1.
Processing of samples. A double swab was directly immersed in the liquid samples, while solid samples were previously crushed in saline buffer. One of the swabs was immediately submitted to Xpert MRSA/SA SSTI assay, while the other was plated. Liquid samples were also inoculated in blood culture bottles. Any single colony was identified, and an antibiogram was performed.
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