NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes - PubMed (original) (raw)
. 2011 Oct 23;8(12):1037-40.
doi: 10.1038/nmeth.1740.
Stefan R Braam, Katerina Koutsis, Elizabeth S Ng, Robert Jenny, Ebba L Lagerqvist, Christine Biben, Tanya Hatzistavrou, Claire E Hirst, Qing C Yu, Rhys J P Skelton, Dorien Ward-van Oostwaard, Sue Mei Lim, Ouda Khammy, Xueling Li, Susan M Hawes, Richard P Davis, Adam L Goulburn, Robert Passier, Owen W J Prall, John M Haynes, Colin W Pouton, David M Kaye, Christine L Mummery, Andrew G Elefanty, Edouard G Stanley
Affiliations
- PMID: 22020065
- DOI: 10.1038/nmeth.1740
NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes
David A Elliott et al. Nat Methods. 2011.
Abstract
NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
Comment in
- Recognizing heart cells in a crowd.
Kamp TJ. Kamp TJ. Nat Methods. 2011 Nov 29;8(12):1013-6. doi: 10.1038/nmeth.1780. Nat Methods. 2011. PMID: 22127217 No abstract available.
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