The bicoid stability factor controls polyadenylation and expression of specific mitochondrial mRNAs in Drosophila melanogaster - PubMed (original) (raw)

Figure 1. Phylogenetic analysis and subcellular localization of BSF.

(A) Phylogenetic tree of the LRPPRC family of proteins. Phylogenetic analyses show two arthropod families related to LRPPRC, i.e. the Bicoid stability factor (BSF) family and the CG14786 family. The BSF family is orthologous to deuterostomian LRPPRC proteins. Dots indicate stable branches by non-parametric bootstrap analysis (found in at least 450 of 500 replicates). Orthologous sequences in nematodes are divergent, leading to long branches. Abbreviations and accession numbers are as follows: H. sapiens, Homo sapiens, NP_573566; M. musculus, Mus musculus, NP_082509; M. domesticus, Monodelphis domesticus, XP_001382190, G. gallus, Gallus gallus, XP_001234903; X. tropicalis, Xenopus tropicalis, NP_001039203; D. rerio, Danio rerio, NP_001136064; S. kowalevskii, Saccoglossus kowaleskii, XP_002734047; S. purpuratus, Strongylocentrotus purpuratus, XP_001200846; B. floridae, Branchiostoma floridae, XP_002613352; A. pisum, Acyrthosiphon pisum, BSF: XP_001944507; D. melanogaster, Drosophila melanogaster, BSF: NP_523596, CG14786: NP_569913; A. gambiae, Anopheles gambiae, BSF: XP_557938, CG14786: XP_321013; C. quinquefasciatus, Culex quinquefasciatus, BSF: XP_001846253; A. aegypti, Aedes aegypti, BSF: XP_001658446, CG14786: XP_001657384; T. castaneum, Tribolium castaneum, BSF: XP_975329. C. elegans, Caenorhabditis elegans, C04E6.11: NP_504540, C14C10.4: NP_506151; B. malayi, Brugia malayi, 52970: XP_001902062, 51715: XP_001901811. (B) S2R+ cells expressing a GFP-tagged BSF-FLAG fusion protein (BSF-FLAG-GFP), counterstained with Mitotracker Deep Red (upper panels), scale bar size 10 µm and HeLa cells expressing a GFP-tagged BSF-FLAG fusion protein (BSF-FLAG-GFP), counterstained with Mitotracker Deep Red (lower panels), scale bar size 10 µm. (C) Western blot analyses of nuclear, mitochondrial and cytoplasmic fractions to determine the subcellular localization of BSF. Antibodies against subunits NDUFS3 of mitochondrial complex I, tubulin and histone H3 were used to assess the purity of the fractions.