Fragments of the HIV-1 Tat protein specifically bind TAR RNA - PubMed (original) (raw)
. 1990 Sep 14;249(4974):1281-5.
doi: 10.1126/science.2205002.
Affiliations
- PMID: 2205002
- DOI: 10.1126/science.2205002
Fragments of the HIV-1 Tat protein specifically bind TAR RNA
K M Weeks et al. Science. 1990.
Abstract
Proteolytically produced carboxyl-terminal fragments of the human immunodeficiency virus type-1 (HIV-1) Tat protein that include a conserved region rich in arginine and lysine bind specifically to transactivation response RNA sequences (TAR). A chemically synthesized 14-residue peptide spanning the basic subdomain also recognizes TAR, identifying this subdomain as central for RNA interaction. TAR RNA forms a stable hairpin that includes a six-residue loop, a trinucleotide pyrimidine bulge, and extensive duplex structure. Competition and interference experiments show that the Tat-derived fragments bind to double-stranded RNA and interact specifically at the pyrimidine bulge and adjacent duplex of TAR.
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