Fragments of the HIV-1 Tat protein specifically bind TAR RNA - PubMed (original) (raw)
. 1990 Sep 14;249(4974):1281-5.
doi: 10.1126/science.2205002.
Affiliations
- PMID: 2205002
- DOI: 10.1126/science.2205002
Fragments of the HIV-1 Tat protein specifically bind TAR RNA
K M Weeks et al. Science. 1990.
Abstract
Proteolytically produced carboxyl-terminal fragments of the human immunodeficiency virus type-1 (HIV-1) Tat protein that include a conserved region rich in arginine and lysine bind specifically to transactivation response RNA sequences (TAR). A chemically synthesized 14-residue peptide spanning the basic subdomain also recognizes TAR, identifying this subdomain as central for RNA interaction. TAR RNA forms a stable hairpin that includes a six-residue loop, a trinucleotide pyrimidine bulge, and extensive duplex structure. Competition and interference experiments show that the Tat-derived fragments bind to double-stranded RNA and interact specifically at the pyrimidine bulge and adjacent duplex of TAR.
Similar articles
- Site-specific cleavage of the transactivation response site of human immunodeficiency virus RNA with a tat-based chemical nuclease.
Jayasena SD, Johnston BH. Jayasena SD, et al. Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3526-30. doi: 10.1073/pnas.89.8.3526. Proc Natl Acad Sci U S A. 1992. PMID: 1565648 Free PMC article. - High affinity binding of TAR RNA by the human immunodeficiency virus type-1 tat protein requires base-pairs in the RNA stem and amino acid residues flanking the basic region.
Churcher MJ, Lamont C, Hamy F, Dingwall C, Green SM, Lowe AD, Butler JG, Gait MJ, Karn J. Churcher MJ, et al. J Mol Biol. 1993 Mar 5;230(1):90-110. doi: 10.1006/jmbi.1993.1128. J Mol Biol. 1993. PMID: 8450553 - A bulge structure in HIV-1 TAR RNA is required for Tat binding and Tat-mediated trans-activation.
Roy S, Delling U, Chen CH, Rosen CA, Sonenberg N. Roy S, et al. Genes Dev. 1990 Aug;4(8):1365-73. doi: 10.1101/gad.4.8.1365. Genes Dev. 1990. PMID: 2227414 - RNA recognition and regulation of HIV-1 gene expression by viral factor Tat.
Naryshkin NA, Gait MJ, Ivanovskaya MG. Naryshkin NA, et al. Biochemistry (Mosc). 1998 May;63(5):489-503. Biochemistry (Mosc). 1998. PMID: 9632883 Review. - Discoveries of Tat-TAR interaction inhibitors for HIV-1.
Yang M. Yang M. Curr Drug Targets Infect Disord. 2005 Dec;5(4):433-44. doi: 10.2174/156800505774912901. Curr Drug Targets Infect Disord. 2005. PMID: 16535863 Review.
Cited by
- Functional comparison of the basic domains of the Tat proteins of human immunodeficiency virus types 1 and 2 in trans activation.
Elangovan B, Subramanian T, Chinnadurai G. Elangovan B, et al. J Virol. 1992 Apr;66(4):2031-6. doi: 10.1128/JVI.66.4.2031-2036.1992. J Virol. 1992. PMID: 1548750 Free PMC article. - Conserved nucleotides in the TAR RNA stem of human immunodeficiency virus type 1 are critical for Tat binding and trans activation: model for TAR RNA tertiary structure.
Delling U, Reid LS, Barnett RW, Ma MY, Climie S, Sumner-Smith M, Sonenberg N. Delling U, et al. J Virol. 1992 May;66(5):3018-25. doi: 10.1128/JVI.66.5.3018-3025.1992. J Virol. 1992. PMID: 1560535 Free PMC article. - Site-specific cleavage of the transactivation response site of human immunodeficiency virus RNA with a tat-based chemical nuclease.
Jayasena SD, Johnston BH. Jayasena SD, et al. Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3526-30. doi: 10.1073/pnas.89.8.3526. Proc Natl Acad Sci U S A. 1992. PMID: 1565648 Free PMC article. - Human chromosome 12 is required for optimal interactions between Tat and TAR of human immunodeficiency virus type 1 in rodent cells.
Alonso A, Derse D, Peterlin BM. Alonso A, et al. J Virol. 1992 Jul;66(7):4617-21. doi: 10.1128/JVI.66.7.4617-4621.1992. J Virol. 1992. PMID: 1602563 Free PMC article. - Peptide models of the Tat-TAR protein-RNA interaction.
Frankel AD. Frankel AD. Protein Sci. 1992 Dec;1(12):1539-42. doi: 10.1002/pro.5560011202. Protein Sci. 1992. PMID: 1304886 Free PMC article. Review. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources