K-fibre minus ends are stabilized by a RanGTP-dependent mechanism essential for functional spindle assembly - PubMed (original) (raw)

. 2011 Nov 13;13(12):1406-14.

doi: 10.1038/ncb2372.

Affiliations

K-fibre minus ends are stabilized by a RanGTP-dependent mechanism essential for functional spindle assembly

Sylvain Meunier et al. Nat Cell Biol. 2011.

Abstract

Chromosome segregation requires the formation of K-fibres, microtubule bundles that attach sister kinetochores to spindle poles. Most K-fibre microtubules originate around the chromosomes through a non-centrosomal RanGTP-dependent pathway and become oriented with the plus ends attached to the kinetochore and the minus ends focused at the spindle poles. The capture and stabilization of microtubule plus ends at the kinetochore has been extensively studied but very little is known on how their minus-end dynamics are controlled. Here we show that MCRS1 is a RanGTP-regulated factor essential for non-centrosomal microtubule assembly. MCRS1 localizes to the minus ends of chromosomal microtubules and K-fibres, where it protects them from depolymerization. Our data reveal the existence of a mechanism that stabilizes the minus ends of chromosomal microtubules and K-fibres, and is essential for the assembly of a functional bipolar spindle.

PubMed Disclaimer

Comment in

References

    1. Science. 2001 Oct 19;294(5542):543-7 - PubMed
    1. J Cell Sci. 2004 Nov 1;117(Pt 23):5461-77 - PubMed
    1. J Cell Biol. 1997 Jun 30;137(7):1567-80 - PubMed
    1. Semin Cell Dev Biol. 2010 May;21(3):276-82 - PubMed
    1. J Cell Biol. 2005 Sep 26;170(7):1057-66 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources