K-fibre minus ends are stabilized by a RanGTP-dependent mechanism essential for functional spindle assembly - PubMed (original) (raw)
. 2011 Nov 13;13(12):1406-14.
doi: 10.1038/ncb2372.
Affiliations
- PMID: 22081094
- DOI: 10.1038/ncb2372
K-fibre minus ends are stabilized by a RanGTP-dependent mechanism essential for functional spindle assembly
Sylvain Meunier et al. Nat Cell Biol. 2011.
Abstract
Chromosome segregation requires the formation of K-fibres, microtubule bundles that attach sister kinetochores to spindle poles. Most K-fibre microtubules originate around the chromosomes through a non-centrosomal RanGTP-dependent pathway and become oriented with the plus ends attached to the kinetochore and the minus ends focused at the spindle poles. The capture and stabilization of microtubule plus ends at the kinetochore has been extensively studied but very little is known on how their minus-end dynamics are controlled. Here we show that MCRS1 is a RanGTP-regulated factor essential for non-centrosomal microtubule assembly. MCRS1 localizes to the minus ends of chromosomal microtubules and K-fibres, where it protects them from depolymerization. Our data reveal the existence of a mechanism that stabilizes the minus ends of chromosomal microtubules and K-fibres, and is essential for the assembly of a functional bipolar spindle.
Comment in
- A new cap for kinetochore fibre minus ends.
Petry S, Vale RD. Petry S, et al. Nat Cell Biol. 2011 Nov 13;13(12):1389-91. doi: 10.1038/ncb2387. Nat Cell Biol. 2011. PMID: 22081093 Free PMC article. - Cytoskeleton. A stabilizing influence for K-fibres.
Bickenson AF. Bickenson AF. Nat Rev Mol Cell Biol. 2011 Dec 14;13(1):2-3. doi: 10.1038/nrm3256. Nat Rev Mol Cell Biol. 2011. PMID: 22166991 No abstract available.
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