Calreticulin as a potential diagnostic biomarker for lung cancer - PubMed (original) (raw)

Fig. 1

Identification and characterization of FMU-CRT 2, 8, 10, 17, 20, and 24 mAbs. A Reactivity of FMU-CRT 2, 8, 10, 17, 20, and 24 with membrane CRT identified by flow cytometric analysis. Jurkat cells were stained with the control mAb (gray histogram) or FMU-CRT 2, 8, 10, 17, 20, 24 (solid line), respectively. B Western blot analysis showed FMU-CRT 2, 8, 10, 17, 20, and 24 could probe the rCRT molecule, control antibody displayed negative reactivity. C Identification of FMU-CRT 2, 8, 10, 17, 20, and 24 mAbs by immunohistochemistry. The paraffin sections of a mixture of CHO cells (CRT negative) and Jurkat cells (CRT positive) were stained with FMU-CRT 2, 8, 10, 17, 20, 24, and the control mAbs, respectively, and visualized by incubation with DAB chromogen. Among these mAbs, FMU-CRT-2 (a), FMU-CRT-8 (b), FMU-CRT-10, and FMU-CRT-17 (data not shown) could be used in immunohistochemistry. FMU-CRT-20 (c), FMU-CRT-24 (data not shown), and the control mAb (d) were immunoreactive-negative. ×400