Evolution of IncA/C blaCMY-₂-carrying plasmids by acquisition of the blaNDM-₁ carbapenemase gene - PubMed (original) (raw)

Evolution of IncA/C blaCMY-₂-carrying plasmids by acquisition of the blaNDM-₁ carbapenemase gene

Alessandra Carattoli et al. Antimicrob Agents Chemother. 2012 Feb.

Abstract

The bla(NDM-1) gene has been reported to be often located on broad-host-range plasmids of the IncA/C type in clinical but also environmental bacteria recovered from the New Delhi, India, area. IncA/C-type plasmids are the main vehicles for the spread of the cephalosporinase gene bla(CMY-2), frequently identified in the United States, Canada, and Europe. In this study, we completed the sequence of IncA/C plasmid pNDM-KN carrying the bla(NDM-1) gene, recovered from a Klebsiella pneumoniae isolate from Kenya. This sequence was compared with those of three IncA/C-type reference plasmids from Escherichia coli, Yersinia ruckeri, and Photobacterium damselae. Comparative analysis showed that the bla(NDM-1) gene was located on a widely diffused plasmid scaffold known to be responsible for the spread of bla(CMY-2)-like genes and consequently for resistance to broad-spectrum cephalosporins. Considering that IncA/C plasmids possess a broad host range, this scaffold might support a large-scale diffusion of the bla(NDM-1) gene among Gram-negative rods.

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Figures

Fig 1

Fig 1

Major structural features of pNDM-KN in comparison with IncA/C plasmids pAR060302 (GenBank accession no. FJ621588), pYR1 (GenBank accession no. CP000602), and pP91278 (GenBank accession no. AB277724). White boxes indicated plasmid scaffold regions that are in common among plasmids. The tra locus is indicated within the boxes. Resistance genes are indicated by orange boxes, except for the _bla_NDM-1 gene, which is indicated by a blue box. Transposon-related genes (tnpA, tnpR, and tnpM) and insertion sequences are indicated by red boxes. Other genes are indicated by boxes colored as follows: violet, the replicase gene repA; gray, restriction enzyme and DNA methylase genes; green, the groEL-groES cluster; pale blue, the sugE and bcl genes; brown, the _bla_CMY genes. hyp, hypothetical.

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