ApoB-100-containing lipoproteins are major carriers of 3-iodothyronamine in circulation - PubMed (original) (raw)

In vitro functional effects of T1AM. A, saturable uptake of [125I]T1AM by LDL receptor-competent HepG2 cells in the absence or presence of LDL (10 μg/ml). Plotted data are the mean ± S.D. of three experiments, each performed in triplicate. B, subcellular localization (membrane and cytoplasm) of [125I]T1AM taken up by HepG2 cells in an LDL-dependent manner after 60 min of incubation. C, saturable uptake of 125I-labeled LDL (10 μg/ml) by LDLR-competent fibroblasts cells at different concentrations of T1AM (0, 10, 40, 80, 160, and 320 p

m

). The data are plotted as fold uptake of 125I-labeled LDL relative to the control (T1AM untreated). D, subcellular localization (surface and intracellular) of 125I-labeled LDL in the presence of T1AM. Human fibroblasts were incubated with 125I-labeled LDL at 37 °C and preincubated with 80 p

m

T1AM, in the presence and absence of a 50-fold excess of unlabeled LDL in DMEM supplemented with 24 m

m

bicarbonate and 10% human LDS at pH 7.4 for 2 h (25). E, effect of T1AM on apoB production by HepG2 cells. HepG2 cells were treated with T1AM or vehicle for 2 days in DMEM supplemented with 10% human LDS (28). F, effect of iproniazid (100 μ

m

) on the production of apoB stimulated by T1AM in HepG2 cells. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ns, not significant.