Reinfection results in accumulation of unintegrated viral DNA in cytopathic and persistent human immunodeficiency virus type 1 infection of CEM cells - PubMed (original) (raw)
Reinfection results in accumulation of unintegrated viral DNA in cytopathic and persistent human immunodeficiency virus type 1 infection of CEM cells
C D Pauza et al. J Exp Med. 1990.
Abstract
High levels of unintegrated viral DNA accumulate during human immunodeficiency virus type 1 (HIV-1) infection of CEM T cells. Reinfection of already infected cells is required to attain these levels and reinfection also promotes the development of HIV-induced cytopathology. Rates of virus production, however, are independent of the accumulation of unintegrated viral DNA. Neutralizing antibody added soon after infection reduced viral DNA levels without appreciably affecting the production of cell-free viral p24 antigen or reverse transcriptase activity. Only 50 pM AZT were required to reduce the accumulation of unintegrated viral DNA by 50% in contrast to the 25 nM required to inhibit virus production by 50%. Cytopathology, as measured by number of syncytia in infected cell cultures, was correlated with highly elevated levels of unintegrated viral DNA. The minimal levels of unintegrated viral DNA present constitutively in the persistently infected HCEM cell line were consonant with the absence of cytopathic effects in these cells. These data demonstrate that inhibiting the reinfection of already infected cells modulates cytopathic HIV-1 infection to a form that is persistent and noncytopathic.
Similar articles
- The cytopathic effect of human immunodeficiency virus is independent of high levels of unintegrated viral DNA accumulated in response to superinfection of cells.
Laurent-Crawford AG, Hovanessian AG. Laurent-Crawford AG, et al. J Gen Virol. 1993 Dec;74 ( Pt 12):2619-28. doi: 10.1099/0022-1317-74-12-2619. J Gen Virol. 1993. PMID: 8277267 - Unintegrated human immunodeficiency virus type 1 DNA in chronically infected cell lines is not correlated with surface CD4 expression.
Besansky NJ, Butera ST, Sinha S, Folks TM. Besansky NJ, et al. J Virol. 1991 May;65(5):2695-8. doi: 10.1128/JVI.65.5.2695-2698.1991. J Virol. 1991. PMID: 2016776 Free PMC article. - Dissociation of unintegrated viral DNA accumulation from single-cell lysis induced by human immunodeficiency virus type 1.
Bergeron L, Sodroski J. Bergeron L, et al. J Virol. 1992 Oct;66(10):5777-87. doi: 10.1128/JVI.66.10.5777-5787.1992. J Virol. 1992. PMID: 1527842 Free PMC article. - Accumulation of unintegrated circular viral DNA in monocytes and growth-arrested T cells following infection with HIV-1.
Sonza S, Kiernan RE, Maerz AL, Deacon NJ, McPhee DA, Crowe SM. Sonza S, et al. J Leukoc Biol. 1994 Sep;56(3):289-93. doi: 10.1002/jlb.56.3.289. J Leukoc Biol. 1994. PMID: 8083601 Review. - The role of unintegrated DNA in HIV infection.
Sloan RD, Wainberg MA. Sloan RD, et al. Retrovirology. 2011 Jul 1;8:52. doi: 10.1186/1742-4690-8-52. Retrovirology. 2011. PMID: 21722380 Free PMC article. Review.
Cited by
- PTEN Mediates the Silencing of Unintegrated HIV-1 DNA.
Phan AT, Zhu Y. Phan AT, et al. Viruses. 2024 Feb 14;16(2):291. doi: 10.3390/v16020291. Viruses. 2024. PMID: 38400066 Free PMC article. - HIV Preintegration Transcription and Host Antagonism.
Wu Y. Wu Y. Curr HIV Res. 2023;21(3):160-171. doi: 10.2174/1570162X21666230621122637. Curr HIV Res. 2023. PMID: 37345240 Free PMC article. - Tat IRES modulator of tat mRNA (TIM-TAM): a conserved RNA structure that controls Tat expression and acts as a switch for HIV productive and latent infection.
Khoury G, Mackenzie C, Ayadi L, Lewin SR, Branlant C, Purcell DFJ. Khoury G, et al. Nucleic Acids Res. 2020 Mar 18;48(5):2643-2660. doi: 10.1093/nar/gkz1181. Nucleic Acids Res. 2020. PMID: 31875221 Free PMC article. - Tat controls transcriptional persistence of unintegrated HIV genome in primary human macrophages.
Meltzer B, Dabbagh D, Guo J, Kashanchi F, Tyagi M, Wu Y. Meltzer B, et al. Virology. 2018 May;518:241-252. doi: 10.1016/j.virol.2018.03.006. Epub 2018 Mar 15. Virology. 2018. PMID: 29549786 Free PMC article. - Long Terminal Repeat Circular DNA as Markers of Active Viral Replication of Human T Lymphotropic Virus-1 in Vivo.
Fox JM, Hilburn S, Demontis MA, Brighty DW, Rios Grassi MF, Galvão-Castro B, Taylor GP, Martin F. Fox JM, et al. Viruses. 2016 Mar 14;8(3):80. doi: 10.3390/v8030080. Viruses. 2016. PMID: 26985903 Free PMC article.
References
- Science. 1984 May 4;224(4648):497-500 - PubMed
- J Mol Biol. 1967 Jun 14;26(2):365-9 - PubMed
- Science. 1984 Aug 24;225(4664):840-2 - PubMed
- Nature. 1984 Dec 20-1985 Jan 2;312(5996):757-60 - PubMed
- Nature. 1984 Dec 20-1985 Jan 2;312(5996):760-3 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources