Saponins from the traditional medicinal plant Momordica charantia stimulate insulin secretion in vitro - PubMed (original) (raw)
Saponins from the traditional medicinal plant Momordica charantia stimulate insulin secretion in vitro
Amy C Keller et al. Phytomedicine. 2011.
Abstract
The antidiabetic activity of Momordica charantia (L.), Cucurbitaceae, a widely-used treatment for diabetes in a number of traditional medicine systems, was investigated in vitro. Antidiabetic activity has been reported for certain saponins isolated from M. charantia. In this study insulin secretion was measured in MIN6 β-cells incubated with an ethanol extract, saponin-rich fraction, and five purified saponins and cucurbitane triterpenoids from M. charantia, 3β,7β,25-trihydroxycucurbita-5,23(E)-dien-19-al (1), momordicine I (2), momordicine II (3), 3-hydroxycucurbita-5,24-dien-19-al-7,23-di-O-β-glucopyranoside (4), and kuguaglycoside G (5). Treatments were compared to incubation with high glucose (27 mM) and the insulin secretagogue, glipizide (50 μM). At 125 μg/ml, an LC-ToF-MS characterized saponin-rich fraction stimulated insulin secretion significantly more than the DMSO vehicle, p=0.02. At concentrations 10 and 25 μg/ml, compounds 3 and 5 also significantly stimulated insulin secretion as compared to the vehicle, p≤0.007, and p=0.002, respectively. This is the first report of a saponin-rich fraction, and isolated compounds from M. charantia, stimulating insulin secretion in an in vitro, static incubation assay.
Copyright © 2011 Elsevier GmbH. All rights reserved.
Conflict of interest statement
Declarations of Interest
The authors have no conflicts of interest to disclose.
Figures
Fig. 1
Compounds 3_β_,7_β_,25-trihydroxycucurbita-5,23(E)-dien-19-al (1), momordicine I (2), momordicine II (3), 3-hydroxycucurbita-5,24-dien-19-al-7,23-di-_O-β_-glucopyranoside (4), kuguaglycoside G (5).
Fig. 2
Insulin secretion activity of the saponin-rich fraction, after 60 min incubation with MIN6 β-cells. The (*) denotes significantly different activity as compared to DMSO vehicle, n=3. Error bars represent standard error.
Fig. 3
Base peak intensity chromatogram (a), derived from a total ion chromatogram, of saponin-rich fraction of M. charantia. Extracted ion chromatogram (b) for a common cucurbitane skeleton, m/z = 437.3290.
Fig. 4
A and B. Insulin secretion activity of compounds 3 and 5, after 60 min incubation with MIN6 β-cells. Positive control is 27 mM glucose together with 50 μM glipizide, and vehicle is DMSO. The (*) denotes significantly different activity as compared to vehicle, n=3. Error bars represent standard error.
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