Four subunits (αβγδ) of the epithelial sodium channel (ENaC) are expressed in the human eye in various locations - PubMed (original) (raw)
Comparative Study
. 2012 Feb 2;53(2):596-604.
doi: 10.1167/iovs.11-8581.
Affiliations
- PMID: 22167092
- DOI: 10.1167/iovs.11-8581
Comparative Study
Four subunits (αβγδ) of the epithelial sodium channel (ENaC) are expressed in the human eye in various locations
Bettina Krueger et al. Invest Ophthalmol Vis Sci. 2012.
Abstract
Purpose: The epithelial sodium channel (ENaC) is typically expressed in sodium-absorbing epithelia. Several reports suggest that ENaC is also expressed in ocular tissues and may play a role in aqueous humor secretion and glaucoma. However, the precise localization of ENaC in the human eye is still unclear. Here, the authors studied ENaC expression in 12 normal human donor eyes and in six eyes of patients with glaucoma.
Methods: Quantitative real-time PCR was used to investigate the expression of α-, β-, γ-, and δ-ENaC transcripts in ocular tissues. In addition, the authors performed immunohistochemical studies using recently generated antibodies against human β- and γ-ENaC.
Results: At the mRNA level, all four ENaC subunits were found to be expressed in a wide range of ocular tissues from normal and glaucomatous human eyes, with the cornea, ciliary body, iris, and retina showing the highest expression levels. At the protein level, β- and γ-ENaC subunits showed distinct distribution patterns and could be immunolocalized primarily to the cell membranes of epithelial cells of the cornea and to the conjunctiva, iris, ciliary body, lens, and retinal pigment epithelium but also to vascular endothelial cells, smooth muscle cells, stromal cells, and retinal neurons. The authors found no altered mRNA level of any subunit in glaucomatous eyes.
Conclusions: All four ENaC subunits (αβγδ) are expressed in the normal human eye, with distinct localization of subunits possibly reflecting different functional states of the channel. The (patho-)physiological roles of ENaC in the various localizations in the eye remain to be determined.
Similar articles
- Clusterin deficiency in eyes with pseudoexfoliation syndrome may be implicated in the aggregation and deposition of pseudoexfoliative material.
Zenkel M, Kruse FE, Jünemann AG, Naumann GO, Schlötzer-Schrehardt U. Zenkel M, et al. Invest Ophthalmol Vis Sci. 2006 May;47(5):1982-90. doi: 10.1167/iovs.05-1580. Invest Ophthalmol Vis Sci. 2006. PMID: 16639006 - Somatostatin receptor gene expression in human ocular tissues: RT-PCR and immunohistochemical study.
Klisovic DD, O'Dorisio MS, Katz SE, Sall JW, Balster D, O'Dorisio TM, Craig E, Lubow M. Klisovic DD, et al. Invest Ophthalmol Vis Sci. 2001 Sep;42(10):2193-201. Invest Ophthalmol Vis Sci. 2001. PMID: 11527930 - Localization of endothelin-1 mRNA expression and immunoreactivity in the anterior segment of human eye: expression of ETA and ETB receptors.
Fernández-Durango R, Rollín R, Mediero A, Roldán-Pallares M, García Feijo J, García Sánchez J, Fernández-Cruz A, Rípodas A. Fernández-Durango R, et al. Mol Vis. 2003 Apr 9;9:103-9. Mol Vis. 2003. PMID: 12692512 - Calcium signalling in ocular tissues: functional activity of G-protein and tyrosine-kinase coupled receptors.
Duncan G, Collison DJ. Duncan G, et al. Exp Eye Res. 2002 Oct;75(4):377-89. Exp Eye Res. 2002. PMID: 12387785 Review. No abstract available. - Ion Channels in the Eye: Involvement in Ocular Pathologies.
Giblin JP, Comes N, Strauss O, Gasull X. Giblin JP, et al. Adv Protein Chem Struct Biol. 2016;104:157-231. doi: 10.1016/bs.apcsb.2015.11.006. Epub 2015 Dec 31. Adv Protein Chem Struct Biol. 2016. PMID: 27038375 Review.
Cited by
- Resting human trabecular meshwork cells experience tonic cation influx.
Yarishkin O, Lakk M, Rudzitis CN, Kirdajova D, Krizaj D. Yarishkin O, et al. Res Sq [Preprint]. 2024 Aug 28:rs.3.rs-4980372. doi: 10.21203/rs.3.rs-4980372/v1. Res Sq. 2024. PMID: 39257996 Free PMC article. Updated. Preprint. - Epithelial Na + Channels Function as Extracellular Sensors.
Kashlan OB, Wang XP, Sheng S, Kleyman TR. Kashlan OB, et al. Compr Physiol. 2024 Mar 29;14(2):1-41. doi: 10.1002/cphy.c230015. Compr Physiol. 2024. PMID: 39109974 Review. - The Variety of Mechanosensitive Ion Channels in Retinal Neurons.
Pang JJ. Pang JJ. Int J Mol Sci. 2024 Apr 30;25(9):4877. doi: 10.3390/ijms25094877. Int J Mol Sci. 2024. PMID: 38732096 Free PMC article. Review. - The Epithelial Sodium Channel-An Underestimated Drug Target.
Lemmens-Gruber R, Tzotzos S. Lemmens-Gruber R, et al. Int J Mol Sci. 2023 Apr 24;24(9):7775. doi: 10.3390/ijms24097775. Int J Mol Sci. 2023. PMID: 37175488 Free PMC article. Review. - The epithelial sodium channel in inflammation and blood pressure modulation.
Ahmad T, Ertuglu LA, Masenga SK, Kleyman TR, Kirabo A. Ahmad T, et al. Front Cardiovasc Med. 2023 Apr 12;10:1130148. doi: 10.3389/fcvm.2023.1130148. eCollection 2023. Front Cardiovasc Med. 2023. PMID: 37123470 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical