Genome-wide analysis of histone H3 acetylation patterns in AML identifies PRDX2 as an epigenetically silenced tumor suppressor gene - PubMed (original) (raw)

. 2012 Mar 8;119(10):2346-57.

doi: 10.1182/blood-2011-06-358705. Epub 2011 Dec 29.

Fabienne Isken, Konstantin Agelopoulos, Hans-Ulrich Klein, Nils H Thoennissen, Gabriele Koehler, Antje Hascher, Nicole Bäumer, Wolfgang E Berdel, Christian Thiede, Gerhard Ehninger, Anke Becker, Peter Schlenke, Yipeng Wang, Michael McClelland, Utz Krug, Steffen Koschmieder, Thomas Büchner, Dae-Yeul Yu, Shailendra Vikram Singh, Klaus Hansen, Hubert Serve, Martin Dugas, Carsten Müller-Tidow

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• PMID: 22207736
• DOI: 10.1182/blood-2011-06-358705

Free article

Genome-wide analysis of histone H3 acetylation patterns in AML identifies PRDX2 as an epigenetically silenced tumor suppressor gene

Shuchi Agrawal-Singh et al. Blood. 2012.

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Abstract

With the use of ChIP on microarray assays in primary leukemia samples, we report that acute myeloid leukemia (AML) blasts exhibit significant alterations in histone H3 acetylation (H3Ac) levels at > 1000 genomic loci compared with CD34(+) progenitor cells. Importantly, core promoter regions tended to have lower H3Ac levels in AML compared with progenitor cells, which suggested that a large number of genes are epigenetically silenced in AML. Intriguingly, we identified peroxiredoxin 2 (PRDX2) as a novel potential tumor suppressor gene in AML. H3Ac was decreased at the PRDX2 gene promoter in AML, which correlated with low mRNA and protein expression. We also observed DNA hypermethylation at the PRDX2 promoter in AML. Low protein expression of the antioxidant PRDX2 gene was clinically associated with poor prognosis in patients with AML. Functionally, PRDX2 acted as inhibitor of myeloid cell growth by reducing levels of reactive oxygen species (ROS) generated in response to cytokines. Forced PRDX2 expression inhibited c-Myc-induced leukemogenesis in vivo on BM transplantation in mice. Taken together, epigenome-wide analyses of H3Ac in AML led to the identification of PRDX2 as an epigenetically silenced growth suppressor, suggesting a possible role of ROS in the malignant phenotype in AML.

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Comment in

• The PRDX2 gene is transcriptionally silenced and de novo methylated in Hodgkin and Reed-Sternberg cells of classical Hodgkin lymphoma.
  Schneider M, Szaumkessel M, Richter J, Ammerpohl O, Hansmann ML, Küppers R, Siebert R, Giefing M. Schneider M, et al. Blood. 2014 Jun 5;123(23):3672-4. doi: 10.1182/blood-2014-02-553263. Blood. 2014. PMID: 24904103 No abstract available.

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