Getting in touch with the clathrin terminal domain - PubMed (original) (raw)

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Getting in touch with the clathrin terminal domain

Sandra K Lemmon et al. Traffic. 2012 Apr.

Abstract

The N-terminal domain (TD) of the clathrin heavy chain is folded into a seven-bladed β-propeller that projects inward from the polyhedral outer clathrin coat. As the most membrane-proximal portion of assembled clathrin, the TD is a major protein-protein interaction node. Contact with the TD β-propeller occurs through short peptide sequences typically located within intrinsically disordered segments of coat components that usually are elements of the membrane-apposed, inner 'adaptor' coat layer. A huge variation in TD-binding motifs is known and now four spatially discrete interaction surfaces upon the β-propeller have been delineated. An important operational feature of the TD interaction sites in vivo is functional redundancy. The recent discovery that 'pitstop' chemical inhibitors apparently occupy only one of the four TD interaction surfaces, but potently block clathrin-mediated endocytosis, warrants careful consideration of the underlying molecular basis for this inhibition.

© 2012 John Wiley & Sons A/S.

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Figures

Figure 1

Figure 1. Clathrin structure and function

A. Stylized cartoon depiction of the clathrin triskelion viewed from the ventral surface. The TD at the N-terminal end of each heavy chain (red) is indicated (gray), while the C-terminal end of each heavy chain is involved in trimerization. B. More accurate schematic representation of the lateral view of a clathrin triskelion based on the high-resolution cryoelectron microscopy structure of the assembled cage (63). The arrow indicates the defined sidedness of the assembled trimer. C. Rapid-freeze deep-etch image of assembled clathrin lattice on the glass-adherent ventral surface of a cultured HeLa cell. In these cells, the clathrin polymer (pseudocolored purple) ranges from large expanses of planar assemblies with a preponderance of hexagonal units through adjacent, pentagon-containing hemispherical-shaped buds, to deeply invaginated structures just about to be released from the plasma membrane as clathrin-coated vesicles. The relative positioning of several individual triskelia (red) interdigitated within the assembled lattice is shown. D. Ribbon diagram of the human clathrin TD (PDB code 2XZG (40)) viewed from the membrane-proximal surface of the β-propeller. The positioning of the seven β-stranded blades is indicated as well as the relative locations of the TD interaction surfaces. Rendered in stick representation are some important side chains (with oxygen in red and nitrogen in blue) defining each binding site (Ile80, Gln89, Phe91 and Lys96 for the clathrin-box LØXØ [DE] site 1/purple; Phe27, Gln152, Ile154 and Ile170 for the W-box site 2/green; Arg188 and Gln192 for the [LI][LI]GXL site 3/yellow; Glu11 for site 4/orange). Note the circumferential positioning of the LØXØ [DE], [LI][LI]GXL and site 4 interaction surfaces. E. Ribbon diagram of the TD β-propeller rotated ~90° to illustrate a lateral view. Color-coded as in D with the relative sidedness (arrows) shown.

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