Recruitment of resting vesicles into recycling pools supports NMDA receptor-dependent synaptic potentiation in cultured hippocampal neurons - PubMed (original) (raw)

A, FM-dye loading protocol (see Fig. 2) used to test the influence of the calcineurin blocker FK506 on potentiation. Bar graph shows ratios (post/pre) for tetanus and tetanus+FK506. Inset (top) shows pre and post FM-dye fluorescence for a synapse treated with FK506. B, SypHy protocol (see Fig. 4) to test the effect of the NO synthase blocker

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-NA and the actin inhibitor jasplakinolide on potentiation. Left, bar graph summarizing recycling fractions for tetanus, tetanus+

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-NA and tetanus+jasplakinolide (Jasp). ANOVA, P < 0.0001; Tukey's: Tet vs. Tet+

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-NA, P < 0.001, Tet _vs._ Tet+Jasp, _P_ > 0.05, Tet+Jasp vs. Tet+

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-NA, P < 0.001, n = 57 synapses from 3 experiments, n = 57 synapses from 3 experiments, n = 76 synapses from 4 experiments, respectively). *P < 0.05, **P < 0.01, ***P < 0.001. Right, sample images of synapses illustrating recycling pool (+stim) and total pool (+NH4Cl) after plasticity in

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-NA and jasplakinolide. C, kymographs generated from an FM1-43-labelled axonal segment demonstrating inhibition of lateral vesicle movement (arrows) after jasplakinolide. Horizontal scale bar, 30 s; vertical bar, 2 μm.