Changes in lipid ordering and state of aggregation in lymphocyte plasma membranes after exposure to mitogens - PubMed (original) (raw)

. 1978 Dec 29;44(3-4):211-32.

doi: 10.1007/BF01944222.

• PMID: 222910
• DOI: 10.1007/BF01944222

Changes in lipid ordering and state of aggregation in lymphocyte plasma membranes after exposure to mitogens

C C Curtain et al. J Membr Biol. 1978.

Abstract

An electron spin probe study was made of the effect of a number of mitogenic agents on the ordering and state of aggregation of the plasma membrane lipids of lymphocytes. These agents, which included phytohemagglutinin, Concanavalin A, the calcium ionophore A23187 and periodate, caused a 20% decrease in lipid ordering in the region of the bilayer probed by 5-nitroxide stearic acid. The corresponding methyl ester probe showed marked probe-probe interaction under the same conditions indicating an aggregation of lipids in the area probed by this label. Studies with mixed lipid vesicles and ganglioside-free cells indicate that these areas are rich in glycolipids capable of hydrogen bonding to the ester probe. The decrease in ordering and the increase in aggregation of the membrane lipids were correlated with the patching and capping of the ligand-receptor complexes. Furthermore, the disappearance of fluorescent ligand from the surface of treated cells corresponded with the return of the spectral parameters of the probes to control cell values. It was concluded that glycolipids might play an important role in ligand-induced cell surface changes either as bearers of receptor groups, as in the case of some gangliosides, or in association by hydrogen-bonding with receptor proteins.

PubMed Disclaimer

Similar articles

• Evidence that mitogenic lectins induce changes in lymphocyte membrane fluidity.
  Barnett RE, Scott RE, Furcht LT, Kersey JH. Barnett RE, et al. Nature. 1974 May 31;249(456):465-6. doi: 10.1038/249465a0. Nature. 1974. PMID: 4365361 No abstract available.
• Incorporation of spin-labeled ganglioside analogues into cell and liposomal membranes.
  Kanda S, Inoue K, Nojima S, Utsumi H, Wiegandt H. Kanda S, et al. J Biochem. 1982 May;91(5):1707-18. doi: 10.1093/oxfordjournals.jbchem.a133862. J Biochem. 1982. PMID: 6284731
• Rapid alterations in cellular morphology and plasma membrane structure induced in rat thymocytes by mitogenic stimuli.
  Hume DA, Thornton MR, Weidemann MJ, Speth V. Hume DA, et al. J Cell Physiol. 1979 Dec;101(3):523-8. doi: 10.1002/jcp.1041010318. J Cell Physiol. 1979. PMID: 118973
• [Interaction of mitogenic lectins with lymphocyte plasma membranes].
  Sitkovskiĭ MV, Shestakova SV, Kozlov IuP. Sitkovskiĭ MV, et al. Biull Eksp Biol Med. 1979 Jul;88(7):89-91. Biull Eksp Biol Med. 1979. PMID: 465726 Russian.

Cited by

• Lipid composition and fluidity of the human immunodeficiency virus envelope and host cell plasma membranes.
  Aloia RC, Tian H, Jensen FC. Aloia RC, et al. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5181-5. doi: 10.1073/pnas.90.11.5181. Proc Natl Acad Sci U S A. 1993. PMID: 8389472 Free PMC article.
• Incorporation of fatty acids by concanavalin A-stimulated lymphocytes and the effect on fatty acid composition and membrane fluidity.
  Calder PC, Yaqoob P, Harvey DJ, Watts A, Newsholme EA. Calder PC, et al. Biochem J. 1994 Jun 1;300 ( Pt 2)(Pt 2):509-18. doi: 10.1042/bj3000509. Biochem J. 1994. PMID: 8002957 Free PMC article.
• Studies on the mechanism of capacitation: albumin-mediated changes in plasma membrane lipids during in vitro incubation of rat sperm cells.
  Davis BK, Byrne R, Bedigian K. Davis BK, et al. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1546-50. doi: 10.1073/pnas.77.3.1546. Proc Natl Acad Sci U S A. 1980. PMID: 6445558 Free PMC article.
• Thermotropic lipid phase separations in human erythrocyte ghosts and cholesterol-enriched rat liver plasma membranes.
  Gordon LM, Mobley PW. Gordon LM, et al. J Membr Biol. 1984;79(1):75-86. doi: 10.1007/BF01868528. J Membr Biol. 1984. PMID: 6330365
• Thermotropic lipid phase separations in human platelet and rat liver plasma membranes.
  Gordon LM, Mobley PW, Esgate JA, Hofmann G, Whetton AD, Houslay MD. Gordon LM, et al. J Membr Biol. 1983;76(2):139-49. doi: 10.1007/BF02000614. J Membr Biol. 1983. PMID: 6315947

References

1. 1. Proc Natl Acad Sci U S A. 1975 Feb;72(2):664-8 - PubMed
2. 1. Biochim Biophys Acta. 1976 Dec 14;455(3):631-43 - PubMed
3. 1. Nature. 1968 Jan 27;217(5126):370-1 - PubMed
4. 1. Eur J Biochem. 1973 Jan 3;32(1):178-87 - PubMed
5. 1. Biochemistry. 1973 Dec 18;12(26):5360-9 - PubMed

MeSH terms

Substances