Use of immunoelectron microscopy to show Ebola virus during the 1989 United States epizootic - PubMed (original) (raw)
Use of immunoelectron microscopy to show Ebola virus during the 1989 United States epizootic
T W Geisbert et al. J Clin Pathol. 1990 Oct.
Abstract
A filovirus, serologically related to Ebola virus, was detected by "post-embedment" immunoelectron microscopical examination of MA-104 cells. These had been infected by inoculation with serum samples obtained during the 1989 epizootic in cynomolgus monkeys (Macaca fascicularis), imported from the Philippines and maintained at Reston, Virginia, USA, a primate holding facility. The immunoelectron microscopy method, when used in conjunction with standard transmission electron microscopy (TEM) of infected cells, provided consistent results and was simple to perform in this epizootic. It is concluded that immunoelectron microscopy is potentially useful in the direct immunological diagnosis of Ebola and related filoviral infections (such as Marburg) in clinical samples obtained from those with acute infection.
Similar articles
- Preliminary report: isolation of Ebola virus from monkeys imported to USA.
Jahrling PB, Geisbert TW, Dalgard DW, Johnson ED, Ksiazek TG, Hall WC, Peters CJ. Jahrling PB, et al. Lancet. 1990 Mar 3;335(8688):502-5. doi: 10.1016/0140-6736(90)90737-p. Lancet. 1990. PMID: 1968529 - Association of Ebola-related Reston virus particles and antigen with tissue lesions of monkeys imported to the United States.
Geisbert TW, Jahrling PB, Hanes MA, Zack PM. Geisbert TW, et al. J Comp Pathol. 1992 Feb;106(2):137-52. doi: 10.1016/0021-9975(92)90043-t. J Comp Pathol. 1992. PMID: 1597531 - Combined simian hemorrhagic fever and Ebola virus infection in cynomolgus monkeys.
Dalgard DW, Hardy RJ, Pearson SL, Pucak GJ, Quander RV, Zack PM, Peters CJ, Jahrling PB. Dalgard DW, et al. Lab Anim Sci. 1992 Apr;42(2):152-7. Lab Anim Sci. 1992. PMID: 1318446 - Reston ebolavirus in humans and animals in the Philippines: a review.
Miranda ME, Miranda NL. Miranda ME, et al. J Infect Dis. 2011 Nov;204 Suppl 3:S757-60. doi: 10.1093/infdis/jir296. J Infect Dis. 2011. PMID: 21987747 Review. - Tissue and cellular tropism, pathology and pathogenesis of Ebola and Marburg viruses.
Martines RB, Ng DL, Greer PW, Rollin PE, Zaki SR. Martines RB, et al. J Pathol. 2015 Jan;235(2):153-74. doi: 10.1002/path.4456. J Pathol. 2015. PMID: 25297522 Review.
Cited by
- Emerging and reemerging infectious diseases: global trends and new strategies for their prevention and control.
Wang S, Li W, Wang Z, Yang W, Li E, Xia X, Yan F, Chiu S. Wang S, et al. Signal Transduct Target Ther. 2024 Sep 11;9(1):223. doi: 10.1038/s41392-024-01917-x. Signal Transduct Target Ther. 2024. PMID: 39256346 Free PMC article. Review. - Diagnostics of Ebola virus.
Bettini A, Lapa D, Garbuglia AR. Bettini A, et al. Front Public Health. 2023 Feb 23;11:1123024. doi: 10.3389/fpubh.2023.1123024. eCollection 2023. Front Public Health. 2023. PMID: 36908455 Free PMC article. Review. - PHILM2Web: A high-throughput database of macromolecular host-pathogen interactions on the Web.
Le TD, Nguyen PD, Korkin D, Thieu T. Le TD, et al. Database (Oxford). 2022 Jun 30;2022:baac042. doi: 10.1093/database/baac042. Database (Oxford). 2022. PMID: 35776535 Free PMC article. - Altered microRNA Transcriptome in Cultured Human Liver Cells upon Infection with Ebola Virus.
Diallo I, Ho J, Laffont B, Laugier J, Benmoussa A, Lambert M, Husseini Z, Soule G, Kozak R, Kobinger GP, Provost P. Diallo I, et al. Int J Mol Sci. 2021 Apr 6;22(7):3792. doi: 10.3390/ijms22073792. Int J Mol Sci. 2021. PMID: 33917562 Free PMC article. - EBOLA VIRUS DISEASE PREPAREDNESS IN COUNTRIES BORDERING DEMOCRATIC REPUBLIC OF CONGO: LESSONS FROM WEST AFRICAN OUTBREAK.
Okoror L, Kapona O, Kasonde M, E TA, Danny A, M OA. Okoror L, et al. Afr J Infect Dis. 2020 Jul 31;14(2):36-41. doi: 10.21010/ajid.v14i2.6. eCollection 2020. Afr J Infect Dis. 2020. PMID: 33884349 Free PMC article.
References
- Can J Microbiol. 1971 Jul;17(7):993-1000 - PubMed
- J Clin Pathol. 1978 Mar;31(3):201-8 - PubMed
- J Histochem Cytochem. 1978 Dec;26(12):1074-81 - PubMed
- Lancet. 1990 Mar 3;335(8688):502-5 - PubMed
- J Med Virol. 1979;4(3):213-25 - PubMed
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical