Application and comparison of large-scale solution-based DNA capture-enrichment methods on ancient DNA - PubMed (original) (raw)

Comparative Study

Application and comparison of large-scale solution-based DNA capture-enrichment methods on ancient DNA

María C Avila-Arcos et al. Sci Rep. 2011.

Abstract

The development of second-generation sequencing technologies has greatly benefitted the field of ancient DNA (aDNA). Its application can be further exploited by the use of targeted capture-enrichment methods to overcome restrictions posed by low endogenous and contaminating DNA in ancient samples. We tested the performance of Agilent's SureSelect and Mycroarray's MySelect in-solution capture systems on Illumina sequencing libraries built from ancient maize to identify key factors influencing aDNA capture experiments. High levels of clonality as well as the presence of multiple-copy sequences in the capture targets led to biases in the data regardless of the capture method. Neither method consistently outperformed the other in terms of average target enrichment, and no obvious difference was observed either when two tiling designs were compared. In addition to demonstrating the plausibility of capturing aDNA from ancient plant material, our results also enable us to provide useful recommendations for those planning targeted-sequencing on aDNA.

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References

    1. Knapp M. & Hofreiter M. Next Generation Sequencing of Ancient DNA: Requirements, Strategies and Perspectives %M doi:10.3390/genes1020227 %U http://www.mdpi.com/2073-4425/1/2/227 Genes %@ 2073–4425 1, 227–243 (2010). - PMC - PubMed
    1. Briggs A. W. et al.. Targeted retrieval and analysis of five Neandertal mtDNA genomes. Science 325, 318–321, 10.1126/science.1174462 (2009). - PubMed
    1. Maricic T., Whitten M. & Paabo S. Multiplexed DNA sequence capture of mitochondrial genomes using PCR products. PLoS One 5, e14004, 10.1371/journal.pone.0014004 (2010). - PMC - PubMed
    1. Hodges E. et al.. Genome-wide in situ exon capture for selective resequencing. Nat Genet 39, 1522–1527, 10.1038/ng.2007.42 (2007). - PubMed
    1. Gnirke A. et al.. Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing. Nat. Biotechnol. 27, 182–189, 10.1038/nbt.1523 (2009). - PMC - PubMed

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