Evolution of the B7 family: co-evolution of B7H6 and NKp30, identification of a new B7 family member, B7H7, and of B7's historical relationship with the MHC - PubMed (original) (raw)

Figure 2

Phylogenetic tree analysis of all members in the B7 family. Deduced aa sequences of V domains are used for this analysis. The bootstrapping tree was constructed using the Neighbor-Joining method with 500 runs (Saitou and Nei 1987). Bootstrapping values are shown at the branch, * indicates less than 100 values. Our analysis consistently showed VISTA (gi24; B7H5) (Wang et al. 2011) to be the most divergent member of B7 family, thus we used it as outgroup. The scale correlates with the divergence time. Accession numbers used for this analysis are: B7.1- human (NP_005182), mouse (CAJ18316). Rat (AAC02262), chicken (CAJ18316); B7.2- human (CAG46642), mouse (P42082), rat (NP_064466), opossum (XP_001371519), chicken (XP_001371519), X. tropicalis (protein ID: 510857 at JGI genome site); B7R- trout (ACH58053), zebrafish (CN017839), minnow (DT293786), stickleback (DN656970), salmon (DW580717), medaka (AM306315); B7H1- human (NP_054862), mouse (EDL41681), rat (XP_001079572), opossum (XP_001371389), chicken (XP_424811, CF251191), fugu (CAF93166), X. tropicalis (protein ID: 16544 at JGI genome site); B7DC- human (AAP13471), mouse (Q9WUL5), rat (EDM13096), opossum (XP_001371408), fugu (CAF93166), X. tropicalis (Protein ID:162957 at JGI genome site), E-shark (AAVX14147467.1, AAVX01029406.1); B7H2- human (BAA31628), mouse (Q9JHJ8), rat (XP_574731), opossum (XP_001377700), chicken (CAA70058), X. laevis (translated from BX848980), X. tropicalis (Protein ID: 170158 at JGI genome site); B7H3- human (AAK15438), mouse (EDL25954), rat (Q7TPB4), opossum (XP_413702), chicken (Q68EV1), X. laevis (XP_700973), zebrafish (XP_001341518), skate (translated from DT378804), E-shark (AAVX01064573.1); B7H4- human (AAP37283), mouse (AAP37284), rat (AAH95842), opossum (XP_001365506), chicken (XP_416546), X. tropicalis (a: CAJ83577; b: protein ID:396923 at JGI genome site), X. laevis (a: AAH41253; b: AAH44000), E-shark (AAVX01001308.1); B7H5- human (AAH20568), Rhesus Monkey (NP_001177807 ), mouse (Q9D659), rat (AAH98723), pig (XP_001928572), cow (AAI23905), X. tropicalis (AAH88869), X. laevis (AAH73319), E- shark (AAVX01038049.1); B7H6- human (CAH18335), cow (NP_001193721), rat (XP_001076811), dog (XP_542518), X. tropicalis (AAI66200), X. laevis (AAH84632), E- shark (AAVX01166257.1); B7HXen- X. tropicalis (AAI61104), X. laevis (AAH85212), B7H7- human (Q9UM44**),** X. tropicalis (AAH96014), X. laevis (ACH85300), E-shark (AAVX01019552.1); TAPBP- chicken (CAA06327), X. tropicalis (XP_002938728), medaka (BAB83851), trout (AAZ66041), salmon (ABO13869), fugu (CAF97838); TAPBPR- X. tropicalis (XP_002941962); Hagfish IGSF3 (BAE93396); X tropicalis unknown B7 family in the scaffold-1303 (Protein ID: 461476). Opossum B7.1 and chicken B7H7 were retrieved from the genomic sequences, NW_001581960 and NW_001471429, respectively. BLAST searches with the V-IgSF of B7H5 (aliases; Gi24, VISTA, C10orf54) (Wang et al. 2011) matched to B7H4 with an E-value ~e−10, but consistently separated from other members of the B7 family with long branch lengths and therefore was used to root the tree. When orthology was not strong, E-shark sequences are shown with accession numbers starting with AAVX. All E-shark sequences are deduced from the genomic scaffolds.